ArticleMolecular biology of the cell2005
Phosphorylation controls CLIMP-63-mediated anchoring of the endoplasmic reticulum to microtubules.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 129 citations in OpenAlex.
- Article
- Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.Biotechnology and bioengineering · 2026Article
- Identification and characterization of a ubiquitin E3 RING ligase of the Chlamydia-like bacterium Simkania negevensis.PLoS pathogens · 2025Article
- Article
- Cytoskeleton-associated protein 4: a double-edged sword in cell growth and aging.Biogerontology · 2025Review
- Cytoskeleton-associated protein 4 affects podocyte cytoskeleton dynamics in diabetic kidney disease.JCI insight · 2025Article
- PERK-dependent reciprocal crosstalk between ER and non-centrosomal microtubules coordinates ER architecture and cell shape.Cell reports · 2025Article
- Sculpting nuclear envelope identity from the endoplasmic reticulum during the cell cycle.Nucleus (Austin, Tex.) · 2024Review
- Cancer cells sense solid stress to enhance metastasis by CKAP4 phase separation-mediated microtubule branching.Cell discovery · 2024Article
- RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress.The EMBO journal · 2023Article
- The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activity.Cell reports · 2023Article
- A tubule-sheet continuum model for the mechanism of nuclear envelope assembly.Developmental cell · 2023Article
- Endoplasmic Reticulum Architecture and Inter-Organelle Communication in Metabolic Health and Disease.Cold Spring Harbor perspectives in biology · 2023Review
- Dynamics of CLIMP-63 S-acylation control ER morphology.Nature communications · 2023Article
- Bi-allelic loss-of-function variants in TMEM147 cause moderate to profound intellectual disability with facial dysmorphism and pseudo-Pelger-Huët anomaly.American journal of human genetics · 2022Article
- Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes.The Journal of cell biology · 2022Article
- Shielding of actin by the endoplasmic reticulum impacts nuclear positioning.Nature communications · 2022Article
- Network organisation and the dynamics of tubules in the endoplasmic reticulum.Scientific reports · 2021Article
- RNA-Binding Profiles of CKAP4 as an RNA-Binding Protein in Myocardial Tissues.Frontiers in cardiovascular medicine · 2021Article
- The coordination of nuclear envelope assembly and chromosome segregation in metazoans.Nucleus (Austin, Tex.) · 2020Review
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microtubule-binding 63-kDa cytoskeleton-linking membrane protein (CLIMP-63) is an integral membrane protein that links the endoplasmic reticulum (ER) to microtubules. Here, we tested whether this interaction is regulated by phosphorylation. Metabolic labeling with (32)P showed that CLIMP-63 is a phosphoprotein with increased phosphorylation during mitosis. CLIMP-63 of mitotic cells is unable to bind to microtubules in vitro. Mitotic phosphorylation can be prevented by mutation of serines 3, 17, and 19 in the cytoplasmic domain of CLIMP-63. When these residues are mutated to glutamic acid, and hence mimic mitotic phosphorylation, CLIMP-63 does no longer bind to microtubules in vitro. Overexpression of the phospho-mimicking mitotic form of CLIMP-63 in interphase cells leads to a collapse of the ER around the nucleus, leaving the microtubular network intact. The results suggest that CLIMP-63-mediated stable anchoring of the ER to microtubules is required to maintain the spatial distribution of the ER during interphase and that this interaction is abolished by phosphorylation of CLIMP-63 during mitosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.