ArticleEukaryotic cell2005
Radiological studies reveal radial differences in the architecture of the polysaccharide capsule of Cryptococcus neoformans.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed, 84 citations in OpenAlex.
- Cell wall melanin impedes growth of theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Neutron Scattering Analysis ofBiomacromolecules · 2024Article
- Electron Microscopy of Cryptococcus neoformans: Processing Challenges to Avoid Artifacts.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Neutron Scattering Analysis ofbioRxiv : the preprint server for biology · 2023Article
- Melanin protects Cryptococcus neoformans from spaceflight effects.Environmental microbiology reports · 2022Article
- Phenotypic Plasticity in the Productions of Virulence Factors Within and Among Serotypes in the Cryptococcus neoformans Species Complex.Mycopathologia · 2022Article
- Influence of Pathogen Carbon Metabolism on Interactions With Host Immunity.Frontiers in cellular and infection microbiology · 2022Review
- The Antimicrobial Peptide MK58911-NHAntimicrobial agents and chemotherapy · 2021Article
- Ultrastructural Study ofFrontiers in microbiology · 2021Article
- ExploringThe Journal of biological chemistry · 2020Article
- Article
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- The capsule ofVirulence · 2019Review
- Characterization and chromium biosorption potential of extruded polymeric substances from Synechococcus mundulus induced by acute dose of gamma irradiation.Environmental science and pollution research international · 2019Article
- Macrolides Inhibit Capsule Formation of Highly VirulentAntimicrobial agents and chemotherapy · 2019Article
- The Buoyancy ofmSphere · 2018Article
- Radioimmunotherapy as a Novel Approach in HIV, Bacterial, and Fungal Infectious Diseases.Cancer biotherapy & radiopharmaceuticals · 2018Review
- Lack of chitin synthase genes impacts capsular architecture and cellular physiology inCell surface (Amsterdam, Netherlands) · 2018Article
- A Predicted Mannoprotein Participates inmSphere · 2018Article
- Dectin-3 Recognizes Glucuronoxylomannan ofFrontiers in immunology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
The polysaccharide capsule of the pathogenic fungus Cryptococcus neoformans is an important virulence factor, but relatively little is known about its architecture. We applied a combination of radiological, chemical, and serological methods to investigate the structure of this polysaccharide capsule. Exposure of C. neoformans cells to gamma radiation, dimethyl sulfoxide, or radiolabeled monoclonal antibody removed a significant part of the capsule. Short intervals of gamma irradiation removed the outer portion of the cryptococcal capsule without killing cells, which could subsequently repair their capsules. Survival analysis of irradiated wild-type, acapsular mutant, and complemented mutant strains demonstrated that the capsule contributed to radioprotection and had a linear attenuation coefficient higher than that of lead. The capsule portions remaining after dimethyl sulfoxide or gamma radiation treatment were comparable in size, 65 to 66 microm3, and retained immunoreactivity for a monoclonal antibody to glucuronoxylomannan. Simultaneous or sequential treatment of the cells with dimethyl sulfoxide and radiation removed the remaining capsule so that it was not visible by light microscopy. The capsule could be protected against radiation by either of the free radical scavengers ascorbic acid and sorbitol. Sugar composition analysis of polysaccharide removed from the outer and inner parts of the capsule revealed significant differences in glucuronic acid and xylose molar ratios, implying differences in the chemical structure of the constituent polysaccharides. Our results provide compelling evidence for the existence of two zones in the C. neoformans capsule that differ in susceptibility to dimethyl sulfoxide and radiation and, possibly, in packing and composition.
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