ArticleEukaryotic cell2005
The function of the coding sequences for the putative pheromone precursors in Podospora anserina is restricted to fertilization.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 74 citations in OpenAlex.
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- A 20-kb lineage-specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum.Molecular plant pathology · 2021Article
- Two mating-type genes MAT1-1-1 and MAT1-1-2 with significant functions in conidiation, stress response, sexual development, and pathogenicity of rice false smut fungus Villosiclava virens.Current genetics · 2020Article
- It's All in the Genes: The Regulatory Pathways of Sexual Reproduction in Filamentous Ascomycetes.Genes · 2019Review
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- Pheromone expression reveals putative mechanism of unisexuality in a saprobic ascomycete fungus.PloS one · 2018Article
- Discovery of long-distance gamete dispersal in a lichen-forming ascomycete.The New phytologist · 2017Article
- Gamete signalling underlies the evolution of mating types and their number.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Article
- A network of HMG-box transcription factors regulates sexual cycle in the fungus Podospora anserina.PLoS genetics · 2013Article
- The role of pheromone receptors for communication and mating in Hypocrea jecorina (Trichoderma reesei).Fungal genetics and biology : FG & B · 2012Article
- ENVOY is a major determinant in regulation of sexual development in Hypocrea jecorina (Trichoderma reesei).Eukaryotic cell · 2012Article
- Sex-specific gene expression during asexual development of Neurospora crassa.Fungal genetics and biology : FG & B · 2012Article
- Presence and functionality of mating type genes in the supposedly asexual filamentous fungus Aspergillus oryzae.Applied and environmental microbiology · 2012Article
- Systematic deletion of homeobox genes in Podospora anserina uncovers their roles in shaping the fruiting body.PloS one · 2012Article
- Fungal mating pheromones: choreographing the dating game.Fungal genetics and biology : FG & B · 2011Review
- Article
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We cloned the pheromone precursor genes of Podospora anserina in order to elucidate their role in the biology of this fungus. The mfp gene encodes a 24-amino-acid polypeptide finished by the CAAX motif, characteristic of fungal lipopeptide pheromone precursors similar to the a-factor precursor of Saccharomyces cerevisiae. The mfm gene encodes a 221-amino-acid polypeptide, which is related to the S. cerevisiae alpha-factor precursor and contains two 13-residue repeats assumed to correspond to the mature pheromone. We deleted the mfp and mfm coding sequence by gene replacement. The mutations specifically affect male fertility, without impairing female fertility and vegetative growth. The male defect is mating type specific: the mat+ Deltamfp and mat- Deltamfm mutants produce male cells inactive in fertilization whereas the mat- Deltamfp and mat+ Deltamfm mutants show normal male fertility. Genetic data indicate that both mfp and mfm are transcribed at a low level in mat+ and mat- vegetative hyphae. Northern-blot analysis shows that their transcription is induced by the mating types in microconidia (mfp by mat+ and mfm by mat-). We managed to cross Deltamfp Deltamfm strains of opposite mating type, by complementation and transient expression of the pheromone precursor gene to trigger fertilization. These crosses were fertile, demonstrating that once fertilization occurs, the pheromone precursor genes are unnecessary for the completion of the sexual cycle. Finally, we show that the constitutively transcribed gpd::mfm and gpd::mfp constructs are repressed at a posttranscriptional level by the noncognate mating type.
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