ArticleEukaryotic cell2005
Multiple metabolic roles for the nonphotosynthetic plastid of the green alga Prototheca wickerhamii.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- High-Quality Genome Assemblies of Two Prototheca wickerhamii Strains.Scientific data · 2026Article
- Herbicide glyphosate efficiently inhibits growth of pathogenicMicrobiology spectrum · 2025Article
- Reduced plastid genomes of colorless facultative pathogens Prototheca (Chlorophyta) are retained for membrane transport genes.BMC biology · 2024Article
- What Happened before Losses of Photosynthesis in Cryptophyte Algae?Molecular biology and evolution · 2022Article
- The Cryptic Plastid ofmSphere · 2020Article
- Comparative Plastid Genomics of Non-Photosynthetic Chrysophytes: Genome Reduction and Compaction.Frontiers in plant science · 2020Article
- Nuclear genome sequence of the plastid-lacking cryptomonad Goniomonas avonlea provides insights into the evolution of secondary plastids.BMC biology · 2018Article
- Comparative analysis of nucleus-encoded plastid-targeting proteins in Rafflesia cantleyi against photosynthetic and non-photosynthetic representatives reveals orthologous systems with potentially divergent functions.Scientific reports · 2018Article
- Reductive evolution of chloroplasts in non-photosynthetic plants, algae and protists.Current genetics · 2018Review
- Multiple losses of photosynthesis and convergent reductive genome evolution in the colourless green algae Prototheca.Scientific reports · 2018Article
- Auxenochlorella protothecoides and Prototheca wickerhamii plastid genome sequences give insight into the origins of non-photosynthetic algae.Scientific reports · 2015Article
- When the lights go out: the evolutionary fate of free-living colorless green algae.The New phytologist · 2015Review
- The Plastid Genome of the Cryptomonad Teleaulax amphioxeia.PloS one · 2015Article
- A plastid without a genome: evidence from the nonphotosynthetic green algal genus Polytomella.Plant physiology · 2014Article
- Article
- The endosymbiotic origin, diversification and fate of plastids.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- The complete plastid genome sequence of the secondarily nonphotosynthetic alga Cryptomonas paramecium: reduction, compaction, and accelerated evolutionary rate.Genome biology and evolution · 2009Article
- A structure-based approach to ligand discovery for 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase: a target for antimicrobial therapy.Journal of medicinal chemistry · 2009Article
- On the origin of chloroplasts, import mechanisms of chloroplast-targeted proteins, and loss of photosynthetic ability - review.Folia microbiologica · 2009Review
- From chloroplasts to "cryptic" plastids: evolution of plastid genomes in parasitic plants.Current genetics · 2008Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of plastids in diverse eukaryotic lineages that have lost the capacity for photosynthesis is well documented. The metabolic functions of such organelles, however, are poorly understood except in the case of the apicoplast in the Apicomplexa, a group of intracellular parasites including Plasmodium falciparum, and the plastid of the green alga Helicosporidium sp., a parasite for which the only host-free stage identified in nature so far is represented by cysts. As a first step in the reconstruction of plastid functions in a nonphotosynthetic, predominantly free-living organism, we searched for expressed sequence tags (ESTs) that correspond to nucleus-encoded plastid-targeted polypeptides in the green alga Prototheca wickerhamii. From 3,856 ESTs, we found that 71 unique sequences (235 ESTs) correspond to different nucleus-encoded putatively plastid-targeted polypeptides. The identified proteins predict that carbohydrate, amino acid, lipid, tetrapyrrole, and isoprenoid metabolism as well as de novo purine biosynthesis and oxidoreductive processes take place in the plastid of P. wickerhamii. Mg-protoporphyrin accumulation and, therefore, plastid-to-nucleus signaling might also occur in this nonphotosynthetic organism, as we identified a transcript which encodes subunit I of Mg-chelatase, the enzyme which catalyzes the first committed step in chlorophyll synthesis. Our data indicate a far more complex metabolism in P. wickerhamii's plastid compared with the metabolic pathways predicted to be located in the apicoplast of P. falciparum and the plastid of Helicosporidium sp.
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