ArticleEukaryotic cell2005
Annotation of genes involved in glycerolipid biosynthesis in Chlamydomonas reinhardtii: discovery of the betaine lipid synthase BTA1Cr.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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65 citing papers in PubMed, 235 citations in OpenAlex.
- Multiomics studies reveal how ambient temperature changes govern cellular responses of Chlamydomonas.The Plant cell · 2026Article
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- Laccaria bicolor adapts to phosphate deficiency at the developmental, transcriptional and metabolic levels.Mycorrhiza · 2025Article
- Comparative Analysis of the Biochemical and Molecular Responses ofMarine drugs · 2024Article
- α/β hydrolase domain-containing protein 1 acts as a lysolipid lipase and is involved in lipid droplet formation.National science review · 2024Article
- Pilot Lipidomics Study of Copepods: Investigation of Potential Lipid-Based Biomarkers for the Early Detection and Quantification of the Biological Effects of Climate Change on the Oceanic Food Chain.Life (Basel, Switzerland) · 2023Article
- Lipid bilayer properties potentially contributed to the evolutionary disappearance of betaine lipids in seed plants.BMC biology · 2023Article
- Inhibition of monogalactosyldiacylglycerol synthesis by down-regulation of MGD1 leads to membrane lipid remodeling and enhanced triacylglycerol biosynthesis in Chlamydomonas reinhardtii.Biotechnology for biofuels and bioproducts · 2022Article
- Diacylglyceryl-N,N,N-trimethylhomoserine-dependent lipid remodeling in a green alga, Chlorella kessleri.Communications biology · 2022Article
- Proteomics unveil a central role for peroxisomes in butyrate assimilation of the heterotrophic Chlorophyte algaFrontiers in microbiology · 2022Article
- Quantitative proteomic comparison of salt stress in Chlamydomonas reinhardtii and the snow alga Chlamydomonas nivalis reveals mechanisms for salt-triggered fatty acid accumulation via reallocation of carbon resources.Biotechnology for biofuels · 2021Article
- The oleaginous astaxanthin-producing alga Chromochloris zofingiensis: potential from production to an emerging model for studying lipid metabolism and carotenogenesis.Biotechnology for biofuels · 2021Review
- Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Dynamism of Metabolic Carbon Flow of Starch and Lipids inFrontiers in plant science · 2021Article
- The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Glyceroglycolipid Metabolism Regulations under Phosphate Starvation Revealed by Transcriptome Analysis inMarine drugs · 2020Article
- Surviving Starvation: Proteomic and Lipidomic Profiling of Nutrient Deprivation in the Smallest Known Free-Living Eukaryote.Metabolites · 2020Article
- Algae: Critical Sources of Very Long-Chain Polyunsaturated Fatty Acids.Biomolecules · 2019Review
- Subcellular Energetics and Carbon Storage inCells · 2019Review
- The bZIP1 Transcription Factor Regulates Lipid Remodeling and Contributes to ER Stress Management inThe Plant cell · 2019Article
5 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid metabolism in flowering plants has been intensely studied, and knowledge regarding the identities of genes encoding components of the major fatty acid and membrane lipid biosynthetic pathways is very extensive. We now present an in silico analysis of fatty acid and glycerolipid metabolism in an algal model, enabled by the recent availability of expressed sequence tag and genomic sequences of Chlamydomonas reinhardtii. Genes encoding proteins involved in membrane biogenesis were predicted on the basis of similarity to proteins with confirmed functions and were organized so as to reconstruct the major pathways of glycerolipid synthesis in Chlamydomonas. This analysis accounts for the majority of genes predicted to encode enzymes involved in anabolic reactions of membrane lipid biosynthesis and compares and contrasts these pathways in Chlamydomonas and flowering plants. As an important result of the bioinformatics analysis, we identified and isolated the C. reinhardtii BTA1 (BTA1Cr) gene and analyzed the bifunctional protein that it encodes; we predicted this protein to be sufficient for the synthesis of the betaine lipid diacylglyceryl-N,N,N-trimethylhomoserine (DGTS), a major membrane component in Chlamydomonas. Heterologous expression of BTA1Cr led to DGTS accumulation in Escherichia coli, which normally lacks this lipid, and allowed in vitro analysis of the enzymatic properties of BTA1Cr. In contrast, in the bacterium Rhodobacter sphaeroides, two separate proteins, BtaARs and BtaBRs, are required for the biosynthesis of DGTS. Site-directed mutagenesis of the active sites of the two domains of BTA1Cr allowed us to study their activities separately, demonstrating directly their functional homology to the bacterial orthologs BtaARs and BtaBRs.
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