ArticleNucleic acids research2005
The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Ferroptosis resistance-related TEP1 as a novel prognostic biomarker involved in immune cell infiltration and tumour progression in glioblastoma.Cancer cell international · 2025Article
- Roles of Small Noncoding Vault RNAs in Intestinal Epithelium Homeostasis and Diseases.International journal of molecular sciences · 2025Review
- M6A Methylase METTL3-mediated downregulation of TEP1 mRNA stability attenuates PDGF-BB-induced HASMC remodeling and inflammation via macrophage polarization.BMC pediatrics · 2025Article
- Major Vault Protein/Lung Resistance-Related Protein: A Novel Biomarker for Inflammation and Acute Infections.Microorganisms · 2024Article
- Structure, Dynamics and Functional Implications of the Eukaryotic Vault Complex.Sub-cellular biochemistry · 2024Review
- Gene polymorphisms associated with sudden decreases in heart rate during extensive peritoneal lavage with distilled water after gastrectomy.World journal of gastrointestinal surgery · 2023Article
- Vault RNA1-1 riboregulates the autophagic function of p62 by binding to lysine 7 and arginine 21, both of which are critical for p62 oligomerization.RNA (New York, N.Y.) · 2022Article
- Review
- 'High vault-age': non-coding RNA control of autophagy.Open biology · 2020Review
- The vault RNA ofThe Journal of biological chemistry · 2019Article
- Article
- Article
- In silico resurrection of the major vault protein suggests it is ancestral in modern eukaryotes.Genome biology and evolution · 2013Article
- Native gel electrophoresis of human telomerase distinguishes active complexes with or without dyskerin.Nucleic acids research · 2012Article
- Structural studies of large nucleoprotein particles, vaults.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2012Review
- Genetic variation in five genes important in telomere biology and risk for breast cancer.British journal of cancer · 2007Article
- DNA damage responses in neural cells: Focus on the telomere.Neuroscience · 2007Review
- The vault exterior shell is a dynamic structure that allows incorporation of vault-associated proteins into its interior.Biochemistry · 2006Article
- Different modes and potencies of translational repression by sequence-specific RNA-protein interaction at the 5'-UTR.Nucleic acids research · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
TEP1 is a protein component of two ribonucleoprotein complexes: vaults and telomerase. The vault-associated small RNA, termed vault RNA (VR), is dependent upon TEP1 for its stable association with vaults, while the association of telomerase RNA with the telomerase complex is independent of TEP1. Both of these small RNAs have been shown to interact with amino acids 1-871 of TEP1 in an indirect yeast three-hybrid assay. To understand the determinants of TEP1-RNA binding, we generated a series of TEP1 deletions and show by yeast three-hybrid assay that the entire Tetrahymena p80 homology region of TEP1 is required for its interaction with both telomerase and VRs. This region is also sufficient to target the protein to the vault particle. Electrophoretic mobility shift assays using the recombinant TEP1 RNA-binding domain (TEP1-RBD) demonstrate that it binds RNA directly, and that telomerase and VRs compete for binding. VR binds weakly to TEP1-RBD in vitro, but mutation of VR sequences predicted to disrupt helices near its central loop enhances binding. Antisense oligonucleotide-directed RNase H digestion of endogenous VR indicates that this region is largely single stranded, suggesting that TEP1 may require access to the VR central loop for efficient binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.