ArticleProceedings of the National Academy of Sciences of the United States of America2005
Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolii.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers, 3 of them syntheses that pooled it.
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Who cites it
116 citing papers in PubMed, 3 syntheses or guidelines pooled it, 463 citations in OpenAlex.
- Plant Salinity Tolerance Conferred by Arbuscular Mycorrhizal Fungi and Associated Mechanisms: A Meta-Analysis.Frontiers in plant science · 2020Pooled it
- Response of Plants to Water Stress: A Meta-Analysis.Frontiers in plant science · 2020Pooled it
- Pooled it
- Screening and Evaluation of Salt-Tolerant Wheat Cultivars.Plants (Basel, Switzerland) · 2026Article
- Regulated Cell Death in Fungi, the Role of Metacaspases and Assay Techniques.Methods and protocols · 2026Review
- Heat-Stress Induced Apoptosis: A New Biotechnological Strategy to Enhance Ganoderic Acids Production inJournal of fungi (Basel, Switzerland) · 2026Article
- Comparative assessment of food-grade osmolytes for enhancing yeast fermentation performance under salt stress.Microbiology spectrum · 2026Article
- Oxidative stress and osmolyte induction caused by a Thiobencarb nano-emulsion in the freshwater alga Chlorella vulgaris.Ecotoxicology (London, England) · 2026Article
- The Effect of Selenium Supplementation on Amino Acid Accumulation by the YeastMolecules (Basel, Switzerland) · 2026Article
- Antiviral potential of crude extracts from two Streptomyces spp. against Cucumber Mosaic Virus infection under greenhouse conditions.Scientific reports · 2025Article
- Proline Tagging for Stress Tolerance in Plants.International journal of genomics · 2025Review
- Plants interfere with non-self recognition of a phytopathogenic fungus via proline accumulation to facilitate mycovirus transmission.Nature communications · 2024Article
- Pro-tumorigenic activity of PYCR1 in gastric cancer through regulating the PI3K/AKT signaling.Heliyon · 2024Article
- Preparation, Structural Identification, and Screening of Egg-Derived Peptides with Facilitating Alcohol Metabolism Activity.Foods (Basel, Switzerland) · 2024Article
- Attenuation of Zucchini mosaic virus disease in cucumber plants by mycorrhizal symbiosis.Plant cell reports · 2024Article
- The Role of Amino Acids in Non-Enzymatic Antioxidant Mechanisms in Cancer: A Review.Metabolites · 2023Review
- Biochemical and metabolomic insights into antifungal mechanism of berberine against Candida glabrata.Applied microbiology and biotechnology · 2023Article
- Overexpression of a WRKY transcription factor McWRKY57-like from Mentha canadensis L. enhances drought tolerance in transgenic Arabidopsis.BMC plant biology · 2023Article
- GC/EI/MS andMetabolites · 2023Article
- An Ascovirus Utilizes Different Types of Host Larval Regulated Cell Death Mechanisms To Produce and Release Vesicles.Journal of virology · 2023Article
56 more citing papers are in PubMed but not listed here.
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2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of reactive oxygen species (ROS) in cell communication, control of gene expression, and oxygen sensing is well established. Inappropriate regulation of ROS levels can damage cells, resulting in a diseased state. In Colletotrichum trifolii, a fungal pathogen of alfalfa, the mutationally activated oncogenic fungal Ras (DARas) elevates levels of ROS, causing abnormal fungal growth and development and eventual apoptotic-like cell death but only when grown under nutrient-limiting conditions. Remarkably, restoration to the wild-type phenotype requires only proline. Here, we describe a generally unrecognized function of proline: its ability to function as a potent antioxidant and inhibitor of programmed cell death. Addition of proline to DARas mutant cells effectively quenched ROS levels and prevented cell death. Treating cells with inhibitors of ROS production yielded similar results. In addition, proline protected wild-type C. trifolii cells against various lethal stresses, including UV light, salt, heat, and hydrogen peroxide. These observations appear to be general because proline also protected yeast cells from lethal levels of the ROS-generating herbicide methyl viologen (paraquat), suggesting a common protective role for proline in response to oxidative stress. The ability of proline to scavenge intracellular ROS and inhibit ROS-mediated apoptosis may be an important and broad-based function of this amino acid in responding to cellular stress, in addition to its well established role as an osmolyte.
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