ArticleMolecular and cellular biology1992
Only two of the five zinc fingers of the eukaryotic transcriptional repressor PRDI-BF1 are required for sequence-specific DNA binding.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Role of PRDM1 in natural killer cells.Molecular biology reports · 2026Review
- Article
- Blimp-1 orchestrates macrophage polarization and metabolic homeostasis via purine biosynthesis in sepsis.Cell death & disease · 2025Article
- Day-night gene expression reveals circadian geneProceedings. Biological sciences · 2024Article
- Functional CRISPR screens in T cells reveal new opportunities for cancer immunotherapies.Molecular cancer · 2024Review
- PRDM1 DNA-binding zinc finger domain is required for normal limb development and is disrupted in split hand/foot malformation.Disease models & mechanisms · 2023Article
- Runx proteins and transcriptional mechanisms that govern memory CD8 T cell development.Immunological reviews · 2021Review
- Conserved and Unique Functions of Blimp1 in Immune Cells.Frontiers in immunology · 2021Review
- NFATc1/αA and Blimp-1 Support the Follicular and Effector Phenotype of Tregs.Frontiers in immunology · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- PD-1 Expression during Acute Infection Is Repressed through an LSD1-Blimp-1 Axis.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- NonO Is a Novel Co-factor of PRDM1 and Regulates Inflammatory Response in Monocyte Derived-Dendritic Cells.Frontiers in immunology · 2020Article
- PRDM1/BLIMP1 is widely distributed to the nascent fetal-placental interface in the mouse gastrula.Developmental dynamics : an official publication of the American Association of Anatomists · 2017Article
- Sequences analyses and expression profiles in tissues and embryos of Japanese flounder (Paralichthys olivaceus) PRDM1.Fish physiology and biochemistry · 2016Article
- SLE-associated risk factors affect DC function.Immunological reviews · 2016Review
- Immunological function of Blimp-1 in dendritic cells and relevance to autoimmune diseases.Immunologic research · 2015Review
- Blimp1/Prdm1 Functions in Opposition to Irf1 to Maintain Neonatal Tolerance during Postnatal Intestinal Maturation.PLoS genetics · 2015Article
- Identification and Characterization of a PRDM14 Homolog in Japanese Flounder (Paralichthys olivaceus).International journal of molecular sciences · 2015Article
- Cellular differentiation regulator BLIMP1 induces Epstein-Barr virus lytic reactivation in epithelial and B cells by activating transcription from both the R and Z promoters.Journal of virology · 2015Article
- Candidate genes of Waldenström's macroglobulinemia: current evidence and research.The application of clinical genetics · 2013Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The eukaryotic transcriptional repressor PRDI-BF1 contains five zinc fingers of the C2H2 type, and the protein binds specifically to PRDI, a 14-bp regulatory element of the beta interferon gene promoter. We have investigated the amino acid sequence requirements for specific binding to PRDI and found that the five zinc fingers and a short stretch of amino acids N terminal to the first finger are necessary and sufficient for PRDI-specific binding. The contribution of individual zinc fingers to DNA binding was investigated by inserting them in various combinations into another zinc finger-containing DNA-binding protein whose own fingers had been removed. We found that insertion of PRDI-BF1 zinc fingers 1 and 2 confer PRDI-binding activity on the recipient protein. In contrast, the insertion of PRDI-BF1 zinc fingers 2 through 5, the insertion of zinc finger 1 or 2 alone, and the insertion of zinc fingers 1 and 2 in reverse order did not confer PRDI-binding activity. We conclude that the first two PRDI-BF1 zinc fingers together are sufficient for the sequence-specific recognition of PRDI.
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