ArticleThe Journal of cell biology2005
Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the microtubule plus end.
Article in The Journal of cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
107 citing papers in PubMed, 195 citations in OpenAlex.
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- A clinical and genotype-phenotype analysis of MACF1 variants.American journal of human genetics · 2025Article
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- EB-SUN, a new microtubule plus-end tracking protein inMolecular biology of the cell · 2024Article
- Liquid-liquid phase separation of microtubule-binding proteins in the regulation of spindle assembly.Cell proliferation · 2024Review
- EB-SUN, a New Microtubule Plus-End Tracking Protein inbioRxiv : the preprint server for biology · 2024Article
- Nuclear elongation during spermiogenesis depends on physical linkage of nuclear pore complexes to bundled microtubules by Drosophila Mst27D.PLoS genetics · 2023Article
- MAPping tubulin mutations.Frontiers in cell and developmental biology · 2023Review
- Multivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends.Nature cell biology · 2023Article
- Stability profile of vimentin rod domain.Protein science : a publication of the Protein Society · 2022Article
- Dynein-dependent collection of membranes defines the architecture and position of microtubule asters in isolated, geometrically confined volumes of cell-free extracts.Molecular biology of the cell · 2022Article
- Deciphering the Contribution of BP230 Autoantibodies in Bullous Pemphigoid.Antibodies (Basel, Switzerland) · 2022Review
- TheMolecular biology of the cell · 2022Article
- Article
- SxIP binding disrupts the constitutive homodimer interface of EB1 and stabilizes EB1 monomer.Biophysical journal · 2021Article
- Strength Through Unity: The Power of the Mega-Scaffold MACF1.Frontiers in cell and developmental biology · 2021Review
- The molecular structure of mammalian primary cilia revealed by cryo-electron tomography.Nature structural & molecular biology · 2020Article
- Arl4D-EB1 interaction promotes centrosomal recruitment of EB1 and microtubule growth.Molecular biology of the cell · 2020Article
- Addressing the Molecular Mechanism of Longitudinal Lamin Assembly Using Chimeric Fusions.Cells · 2020Article
- Article
47 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
EB1 is a member of a conserved protein family that localizes to growing microtubule plus ends. EB1 proteins also recruit cell polarity and signaling molecules to microtubule tips. However, the mechanism by which EB1 recognizes cargo is unknown. Here, we have defined a repeat sequence in adenomatous polyposis coli (APC) that binds to EB1's COOH-terminal domain and identified a similar sequence in members of the microtubule actin cross-linking factor (MACF) family of spectraplakins. We show that MACFs directly bind EB1 and exhibit EB1-dependent plus end tracking in vivo. To understand how EB1 recognizes APC and MACFs, we solved the crystal structure of the EB1 COOH-terminal domain. The structure reveals a novel homodimeric fold comprised of a coiled coil and four-helix bundle motif. Mutational analysis reveals that the cargo binding site for MACFs maps to a cluster of conserved residues at the junction between the coiled coil and four-helix bundle. These results provide a structural understanding of how EB1 binds two regulators of microtubule-based cell polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.