ArticleJournal of clinical microbiology2005
In vitro method to study antifungal perfusion in Candida biofilms.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect againstJournal of fungi (Basel, Switzerland) · 2020Review
- Pathogenesis and Clinical Relevance ofFrontiers in microbiology · 2020Review
- Delineating the Biofilm Inhibition Mechanisms of Phenolic and Aldehydic Terpenes againstACS omega · 2019Article
- ZCF32, a fungus specific Zn(II)2 Cys6 transcription factor, is a repressor of the biofilm development in the human pathogen Candida albicans.Scientific reports · 2016Article
- The Host's Reply to Candida Biofilm.Pathogens (Basel, Switzerland) · 2016Review
- Candida Biofilms: Development, Architecture, and Resistance.Microbiology spectrum · 2015Review
- Novel Nystatin A₁ derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis.Medical microbiology and immunology · 2014Article
- Fungal biofilms, drug resistance, and recurrent infection.Cold Spring Harbor perspectives in medicine · 2014Review
- Micafungin at physiological serum concentrations shows antifungal activity against Candida albicans and Candida parapsilosis biofilms.Antimicrobial agents and chemotherapy · 2014Article
- Candida species biofilm and Candida albicans ALS3 polymorphisms in clinical isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Preventing root caries development under oral biofilm challenge in an artificial mouth.Medicina oral, patologia oral y cirugia bucal · 2013Article
- Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries.Annals of clinical microbiology and antimicrobials · 2013Article
- Interface of Candida albicans biofilm matrix-associated drug resistance and cell wall integrity regulation.Eukaryotic cell · 2011Article
- UDP-glucose 4, 6-dehydratase activity plays an important role in maintaining cell wall integrity and virulence of Candida albicans.PLoS pathogens · 2011Article
- Optimizing a Candida biofilm microtiter plate model for measurement of antifungal susceptibility by tetrazolium salt assay.Journal of clinical microbiology · 2011Article
- Antimicrobial activity of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study.International journal of oral and maxillofacial surgery · 2010Article
- Interaction of Candida albicans biofilms with antifungals: transcriptional response and binding of antifungals to beta-glucans.Antimicrobial agents and chemotherapy · 2010Article
- Antifungal susceptibility of Candida albicans biofilms on titanium discs with different surface roughness.Clinical oral investigations · 2007Article
- Role for cell density in antifungal drug resistance in Candida albicans biofilms.Antimicrobial agents and chemotherapy · 2007Article
- Importance of biofilm in Candida parapsilosis and evaluation of its susceptibility to antifungal agents by colorimetric method.Folia microbiologica · 2007Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antimycotic perfusion through Candida biofilms was demonstrated by a modification of a simple in vitro diffusion cell bioassay system. Using this model, the perfusion of three commonly used antifungal agents, amphotericin B, fluconazole, and flucytosine, was investigated in biofilms of three different Candida species (i.e., Candida albicans, Candida parapsilosis, and Candida krusei) that were developed on microporous filters. Scanning electron microscopy revealed that C. albicans formed a contiguous biofilm with tightly packed blastospores and occasional hyphae compared with C. parapsilosis and C. krusei, which developed confluent biofilms displaying structural heterogeneity and a lesser cell density, after 48 h of incubation on nutrient agar. Minor structural changes were also perceptible on the superficial layers of the biofilm after antifungal perfusion. The transport of antifungals to the distal biofilm-substratum interface was most impeded by C. albicans biofilms in comparison to C. parapsilosis and C. krusei. Fluconazole and flucytosine demonstrated similar levels of perfusion, while amphotericin B was the least penetrant through all three biofilms, although the latter appeared to cause the most structural damage to the superficial cells of the biofilm compared with the other antifungals. These results suggest that the antifungal perfusion through biofilm mode of growth in Candida is dependent both on the antimycotic and the Candida species in question, and in clinical terms, these phenomena could contribute to the failure of Candida biofilm-associated infections. Finally, the in vitro model we have described should serve as a useful system to investigate the complex interactions that appear to operate in vivo within the biofilm-antifungal interphase.
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Registered trials
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