ArticleJournal of clinical microbiology2005
Self-assembly of the recombinant capsid protein of a bovine norovirus (BoNV) into virus-like particles and evaluation of cross-reactivity of BoNV with human noroviruses.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Molecular characteristics of bovine norovirus and nebovirus in Swedish dairy herds.Acta veterinaria Scandinavica · 2025Article
- Animals as Reservoir for Human Norovirus.Viruses · 2019Review
- Phosphatidylinositol 3-Kinase/Akt and MEK/ERK Signaling Pathways Facilitate Sapovirus Trafficking and Late Endosomal Acidification for Viral Uncoating in LLC-PK Cells.Journal of virology · 2018Article
- Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens.Journal of virology · 2018Article
- Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures.The Journal of general virology · 2017Article
- Bovine noroviruses: A missing component of calf diarrhoea diagnosis.Veterinary journal (London, England : 1997) · 2016Review
- Pathogenesis of GIII.2 bovine norovirus, CV186-OH/00/US strain in gnotobiotic calves.Veterinary microbiology · 2014Article
- Retrospective serosurveillance of bovine norovirus (GIII.2) and nebovirus in cattle from selected feedlots and a veal calf farm in 1999 to 2001 in the United States.Archives of virology · 2014Article
- Exposure to human and bovine noroviruses in a birth cohort in southern India from 2002 to 2006.Journal of clinical microbiology · 2013Article
- Characterization of emerging GII.g/GII.12 noroviruses from a gastroenteritis outbreak in the United States in 2010.Journal of clinical microbiology · 2011Article
- Bovine norovirus: carbohydrate ligand, environmental contamination, and potential cross-species transmission via oysters.Applied and environmental microbiology · 2010Article
- Complete genomic characterization and antigenic relatedness of genogroup III, genotype 2 bovine noroviruses.Archives of virology · 2007Article
- Pathogenesis of a genogroup II human norovirus in gnotobiotic pigs.Journal of virology · 2006Article
- Porcine noroviruses related to human noroviruses.Emerging infectious diseases · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
None of the enteric caliciviruses except Po/Sapo/GIII/Cowden/80/US replicates in cell culture, which complicates efforts to develop control strategies or to study viral replication. To develop serological assays for bovine noroviruses (BoNVs) and to determine the cross-reactivity of BoNV with human noroviruses, we generated two recombinant baculoviruses, rCV186-OH and rJNCV, to express the capsid genes of Bo/CV186-OH/00/US (Norovirus genogroup III [GIII], genotype 2 [GIII/2]). rCV186-OH expressed the expected 57-kDa capsid protein, but rJNCV expressed a truncated capsid protein of 35 kDa. Sequence analysis of rJNCV identified a single nucleotide deletion in the P domain of the capsid gene, which introduced a stop codon at amino acid 323. The recombinant capsid protein produced by rCV186-OH but not that produced by rJNCV self-assembled into virus-like particles (VLPs) similar to native BoNV. An antibody-capture enzyme-linked immunosorbent assay (ELISA) and antigen-capture ELISA (Ag-ELISA) detected serum antibody and antigen, respectively, from calves infected with Bo/CV186-OH/00/US but not antibodies or antigens to other enteric viruses. In other tests of the GIII/2 BoNV Ag-ELISA, no cross-reactivity was observed with VLPs from one GI and four GII human noroviruses and porcine sapovirus Cowden strain. Because, like human noroviruses, BoNVs do not grow in cell culture, the BoNV VLPs will be useful in the serological assays described for the detection of BoNV antibody and antigen. Consistent with the phylogenetic analysis of the capsid genes of bovine and human noroviruses (M. G. Han, J. R. Smiley, C. Thomas, and L. J. Saif, J. Clin. Microbiol. 42:5214-5224, 2004), the results suggest that GIII/2 BoNV does not share significant antigenic relationships with the five characterized human noroviruses tested.
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