ArticleJournal of clinical microbiology2005
Duplex reverse transcription-PCR followed by nested PCR assays for detection and identification of Brazilian alphaviruses and flaviviruses.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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Who cites it
74 citing papers in PubMed.
- A New Threat on the Rise: Oropouche Viral Infection.Annals of African medicine · 2026Review
- Mutations in the NS5 RdRp Domain of Zika and Dengue Viruses: Insights into Molecular Patterns in Inland Midwestern Brazil.Tropical medicine and infectious disease · 2026Article
- Eco-epidemiology of arbovirus infections among non-human primates in Southeastern Brazil.PLoS neglected tropical diseases · 2025Article
- Revisiting the emergence of the Chikungunya virus in Alagoas, Northeast of Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Article
- Bussuquara Virus: A Neglected Orthoflavivirus with Broad Distribution Across Central and South America and the Caribbean.Viruses · 2025Review
- Article
- Mayaro Virus: An Emerging Alphavirus in the Americas.Viruses · 2024Review
- Cryptic circulation of chikungunya virus in São Jose do Rio Preto, Brazil, 2015-2019.PLoS neglected tropical diseases · 2024Article
- Arbovirus surveillance in mosquitoes: Historical methods, emerging technologies, and challenges ahead.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- A geopositioned and evidence-graded pan-species compendium of Mayaro virus occurrence.Scientific data · 2023Article
- Arboviral Infections in Neurological Disorders in Hospitalized Patients in São José do Rio Preto, São Paulo, Brazil.Viruses · 2022Article
- Chikungunya, Zika, Mayaro, and Equine Encephalitis virus detection in adult Culicinae from South Central Mato Grosso, Brazil, during the rainy season of 2018.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Article
- Rocio Virus: An Updated View on an Elusive Flavivirus.Viruses · 2021Review
- Orthobunyaviruses in the Caribbean: Melao and Oropouche virus infections in school children in Haiti in 2014.PLoS neglected tropical diseases · 2021Article
- Article
- Dengue-2 and Guadeloupe Mosquito Virus RNA Detected inInsects · 2021Article
- Arbovirus, herpesvirus, and enterovirus associated with neurological syndromes in adult patients of a university hospital, 2017-2018.Revista da Sociedade Brasileira de Medicina Tropical · 2021Article
- Fatal Outcome of Ilheus Virus in the Cerebrospinal Fluid of a Patient Diagnosed with Encephalitis.Viruses · 2020Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new approach was developed for the rapid detection and identification of Brazilian alphaviruses and flaviviruses. The methodology involves the genus-specific detection of Alphavirus and Flavivirus by a duplex reverse transcription-PCR (D-RT-PCR), followed by multiplex nested PCR (M-N-PCR) or nested PCR (N-PCR) assays for species-specific identification. By this protocol, 25 arboviruses were specifically detected and identified. Detection levels between 10(1.3) and 10(3.5) 50% tissue culture infective doses (TCID(50))/ml of Flavivirus and Alphavirus strains were achieved by D-RT-PCR, and levels of <1 TCID(50)/ml were achieved by M-N-PCR assays. To assess the suitability and clinical application of this methodology, a total of 101 human or animal stored samples were analyzed. Results obtained suggest that this technique could be applied as a rapid diagnostic tool in clinical samples in which arbovirus infection is suspected and differential diagnosis is required, avoiding the need to test specimens by separate PCR methods.
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