ArticleJournal of clinical microbiology2005
Use of PCR targeting of internal transcribed spacer regions and single-stranded conformation polymorphism analysis of sequence variation in different regions of rrna genes in fungi for rapid diagnosis of mycotic keratitis.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 119 citations in OpenAlex.
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- Antibacterial potential of marine sponge-associated fungus Amesia atrobrunnea strain AF5 against multidrug-resistant Gram-positive bacteria.Archives of microbiology · 2026Article
- Preliminary Characterization of Skin Microbiota and Mycobiota in Atopic Dermatitis by Metagenomic and Culture-Based Analyses.Life (Basel, Switzerland) · 2026Article
- Comparative study of triterpene compounds in different processedFood chemistry: X · 2026Article
- Assessment of glycanolytic enzymes producing potentialities of three strains of Penicillium sp.Biotechnology letters · 2026Article
- Genomic and biological characterization of Streptomyces strains isolated from barley.BMC microbiology · 2025Article
- The native soil microbiome is critical for early root-associated microbiota assembly and canola growth.Environmental microbiome · 2025Article
- Characterization of fungal communities in Puerto Rican caves using internal transcribed spacer sequencing.Microbiology resource announcements · 2025Article
- Toxicity Assay and Pathogenic Process Analysis ofMicroorganisms · 2025Article
- Characterization of intestinal fungal communities through ITS amplicon from metagenomic libraries of sea cucumber (Microbiology resource announcements · 2025Article
- Rare Chlorinated Fungal Metabolite and Alpha-Pyrones from an Endophytic Fungus Nigrospora sp.ACS omega · 2025Article
- Isolation and Identification of Apiospora intestini from Hedera nepalensis Leaf Blight and Determination of Antagonism of Phomopsis sp.Current microbiology · 2025Article
- Renaissance of an Emerging Pathogen-Clinical ophthalmology (Auckland, N.Z.) · 2025Article
- Endophthalmitis patients in Makassar City: molecular identification of pathogenic fungal profile.BMC infectious diseases · 2024Observational
- Screening of the Biocontrol Efficacy of PotentMicroorganisms · 2024Article
- Emergence of Clonally-Related South Asian Clade I Clinical Isolates ofJournal of fungi (Basel, Switzerland) · 2023Article
- Diagnosis of Aspergillosis in Horses.Journal of fungi (Basel, Switzerland) · 2023Article
- Fusarium spp. associated with Chenopodium quinoa crops in Colombia.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increased incidence of fungal infections in the recent past has been attributed to the increase in the number of human immunodeficiency virus-positive and AIDS patients. Early diagnosis of mycoses in patients is crucial for prompt antifungal therapy. Immunological methods of diagnosis have not been found to be satisfactory, and recent research has been diverted to the use of PCR for the sensitive and early diagnosis at the molecular level. In the present study we targeted different regions of the rRNA gene to diagnose cases of mycotic keratitis and identify the causal agents. Six fungus-specific primers (primers ITS1, ITS2, ITS3, ITS4, invSR1R, and LR12R) were used, and the amplified products were analyzed by single-stranded conformation polymorphism (SSCP) analysis. Dendrograms of these SSCP patterns, prepared on the basis of Jaccard's coefficient, indicated that the PCR products obtained with primer pair ITS1 and ITS2 were the best for the identification of fungi. The results were confirmed by sequencing of the PCR products, and the approach was successfully tested experimentally for the detection of mycotic keratitis caused by Aspergillus fumigatus and was used for the diagnosis of fungal corneal ulcers in patients.
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