ArticleBiophysical journal2005
New fluorescence correlation spectroscopy enabling direct observation of spatiotemporal dependence of diffusion constants as an evidence of anomalous transport in extracellular matrices.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Simulation Studies of Dynamical Heterogeneity in a Dense Two-Dimensional Dimer-Solvent System with Obstacles.Entropy (Basel, Switzerland) · 2024Article
- Mechanobiology of 3D cell confinement and extracellular crowding.Biophysical reviews · 2024Review
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy.Nature communications · 2022Article
- Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation spectroscopy.Scientific reports · 2021Article
- Anomalous Diffusion Inside Soft Colloidal Suspensions Investigated by Variable Length Scale Fluorescence Correlation Spectroscopy.ACS omega · 2020Article
- Anomalous Diffusion in Inverted Variable-Lengthscale Fluorescence Correlation Spectroscopy.Biophysical journal · 2019Article
- Spot variation fluorescence correlation spectroscopy by data post-processing.Scientific reports · 2017Article
- The Secreted Signaling Protein Wnt3 Is Associated with Membrane Domains In Vivo: A SPIM-FCS Study.Biophysical journal · 2016Article
- A straightforward approach for gated STED-FCS to investigate lipid membrane dynamics.Methods (San Diego, Calif.) · 2015Article
- Particle transport through hydrogels is charge asymmetric.Biophysical journal · 2015Article
- Fluorescent correlation spectroscopy measurements with adaptive optics in the intercellular space of spheroids.Biomedical optics express · 2014Article
- Measurement of biomolecular diffusion in extracellular matrix condensed by fibroblasts using fluorescence correlation spectroscopy.PloS one · 2013Article
- Fluorescence correlation spectroscopy: past, present, future.Biophysical journal · 2011Review
- Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes.Biophysical journal · 2011Article
- Near-critical fluctuations and cytoskeleton-assisted phase separation lead to subdiffusion in cell membranes.Biophysical journal · 2011Article
- Physiological environment induces quick response - slow exhaustion reactions.Frontiers in physiology · 2011Article
- Diffusion and partitioning of fluorescent lipid probes in phospholipid monolayers.Biophysical journal · 2009Article
- Measuring diffusion of lipid-like probes in artificial and natural membranes by raster image correlation spectroscopy (RICS): use of a commercial laser-scanning microscope with analog detection.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Anisotropic diffusion of fluorescently labeled ATP in rat cardiomyocytes determined by raster image correlation spectroscopy.American journal of physiology. Cell physiology · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potential of fluorescence correlation spectroscopy (FCS) is extended to enable the direct observation of anomalous subdiffusion (ASD) in inhomogeneous media that are of great importance particularly in many biological systems, such as membranes, cytoplasm, and extracellular matrices (ECMs). Because ASD can be confirmed by monitoring the spatiotemporal dependence of observable diffusion coefficients (D(obs)), the size of the effective confocal volume (V(eff)) for FCS sampling (sampling volume) was continuously changed on a scale of 300-500 nm using a motorized variable beam expander through which an illuminating laser beam passes. This new method, namely, sampling-volume-controlled (SVC)-FCS, was applied to the analysis of hyaluronan (HA) aqueous solutions where the D(obs) of light-emitting solute (Alexa 488) markedly changed, corresponding to the change in V(eff) (220-340 nm in the half-axis), because the network structure of HA of 7-33 nm (nanostructure) interferes with the material transport within it. The results indicate that moderate ASD may occur even in the presence of a small amount ( approximately 0.1 wt %) of HA in ECM. Because the change in D(obs) along with the traveling distance (the mean-square displacement) can be identified even in systems with no deformation of the autocorrelation function, this technique has a great potential for general applications to many biological systems in which ASD shows complex time and space dependences.
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