ArticleProceedings of the National Academy of Sciences of the United States of America2005
A universal plasmid library encoding all permutations of small interfering RNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 39 citations in OpenAlex.
- Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli.MicrobiologyOpen · 2019Article
- Circular siRNAs for Reducing Off-Target Effects and Enhancing Long-Term Gene Silencing in Cells and Mice.Molecular therapy. Nucleic acids · 2018Article
- Caged circular siRNAs for photomodulation of gene expression in cells and mice.Chemical science · 2018Article
- Targeting Human Telomerase Reverse Transcriptase by a Simple siRNA Expression Cassette in HepG2 Cells.Hepatitis monthly · 2015Article
- Introduction of mismatches in a random shRNA-encoding library improves potency for phenotypic selection.PloS one · 2014Article
- Identification of GAS1 as an epirubicin resistance-related gene in human gastric cancer cells with a partially randomized small interfering RNA library.The Journal of biological chemistry · 2009Article
- RNA modulators of complex phenotypes in mammalian cells.PloS one · 2009Article
- Article
- RNA-mediated gene silencing in hematopoietic cells.Journal of biomedicine & biotechnology · 2006Article
- Vector-based siRNA delivery strategies for high-throughput screening of novel target genes.Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research · 2005Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small interfering RNA (siRNA) is normally designed to silence preselected known genes. Such selections are inevitably prone to bias as a result of limited knowledge about the biological process, transcript identity, and functions. A library that contains all permutations of siRNA could avoid such problems. In this paper, it is shown that 5 x 10(7) siRNA-encoding plasmids can be constructed in a single tube by using vectors with two mutated RNA polymerase III promoters arranged in a convergent manner. Such a library was used to carry out genomewide screening of functional genes in a phenotype-driven manner. Multiple siRNAs that induce a significant increase of cell proliferation speed were identified.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.