ArticleProceedings of the National Academy of Sciences of the United States of America2005
Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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Who cites it
33 citing papers in PubMed, 69 citations in OpenAlex.
- SETDB1/ATF7IP regulate the precise genome engineering of HUSH-regulated genes.Epigenetics & chromatin · 2026Article
- Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells.Scientific reports · 2025Article
- Unraveling N-Terminal Proteoform Interactomes via Multiplexed Recombineering in Salmonella.Methods in molecular biology (Clifton, N.J.) · 2025Article
- On the Origins of Homology Directed Repair in Mammalian Cells.International journal of molecular sciences · 2021Article
- Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics.Frontiers in microbiology · 2020Review
- Gene Correction of Point Mutations Using PolyPurine Reverse Hoogsteen Hairpins Technology.Frontiers in genome editing · 2020Review
- Genome-wide Mapping of Off-Target Events in Single-Stranded Oligodeoxynucleotide-Mediated Gene Repair Experiments.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Mechanisms of precise genome editing using oligonucleotide donors.Genome research · 2017Article
- Replicative DNA polymerase δ but not ε proofreads errors in Cis and in Trans.PLoS genetics · 2015Article
- Stimulation of oligonucleotide-directed gene correction by Redβ expression and MSH2 depletion in human HT1080 cells.Molecules and cells · 2015Article
- Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems.PloS one · 2015Article
- Combinatorial gene editing in mammalian cells using ssODNs and TALENs.Scientific reports · 2014Article
- Oligonucleotide delivery by nucleofection does not rescue the reduced proliferation phenotype of gene-edited cells.Nucleic acid therapeutics · 2012Article
- DNA damage response pathway and replication fork stress during oligonucleotide directed gene editing.Molecular therapy. Nucleic acids · 2012Article
- Stable gene targeting in human cells using single-strand oligonucleotides with modified bases.PloS one · 2012Article
- Strand bias influences the mechanism of gene editing directed by single-stranded DNA oligonucleotides.Nucleic acids research · 2011Article
- Progress and prospects: oligonucleotide-directed gene modification in mouse embryonic stem cells: a route to therapeutic application.Gene therapy · 2011Review
- Subtle gene modification in mouse ES cells: evidence for incorporation of unmodified oligonucleotides without induction of DNA damage.Nucleic acids research · 2010Article
- Parameters of oligonucleotide-mediated gene modification in mouse ES cells.Journal of cellular and molecular medicine · 2010Article
- Adeno-associated virus gene repair corrects a mouse model of hereditary tyrosinemia in vivo.Hepatology (Baltimore, Md.) · 2010Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted gene modification mediated by single-stranded oligonucleotides (SSOs) holds great potential for widespread use in a number of biological and biomedical fields, including functional genomics and gene therapy. By using this approach, specific genetic changes have been created in a number of prokaryotic and eukaryotic systems. In mammalian cells, the precise mechanism of SSO-mediated chromosome alteration remains to be established, and there have been problems in obtaining reproducible targeting efficiencies. It has previously been suggested that the chromatin structure, which changes throughout the cell cycle, may be a key factor underlying these variations in efficiency. This hypothesis prompted us to systematically investigate SSO-mediated gene repair at various phases of the cell cycle in a mammalian cell line. We found that the efficiency of SSO-mediated gene repair was elevated by approximately 10-fold in thymidine-treated S-phase cells. The increase in repair frequency correlated positively with the duration of SSO/thymidine coincubation with host cells after transfection. We supply evidence suggesting that these increased repair frequencies arise from a thymidine-induced slowdown of replication fork progression. Our studies provide fresh insight into the mechanism of SSO-mediated gene repair in mammalian cells and demonstrate how its efficiency may be reliably and substantially increased.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.