ArticleProceedings of the National Academy of Sciences of the United States of America2005
Stretched exponential decay and correlations in the catalytic activity of fluctuating single lipase molecules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 293 citations in OpenAlex.
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- Single molecule-driven nanomotors reveal the dynamic-disordered chemomechanical transduction of active enzymes.Science advances · 2025Article
- Memory effects of transcription regulator-DNA interactions in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Watching a Single Enzyme at Work Using Single-Molecule Surface-Enhanced Raman Scattering and DNA Origami-Based Plasmonic Antennas.ACS nano · 2024Article
- Optical sequencing of single synthetic polymers.Nature chemistry · 2024Article
- Dynamic Heterogeneity in the Optical Signals from Single Nano-Objects.The journal of physical chemistry. B · 2023Review
- Theoretical Tools to Quantify Stochastic Fluctuations in Single-Molecule Catalysis by Enzymes and Nanoparticles.ACS omega · 2022Review
- Non-ergodicity of a globular protein extending beyond its functional timescale.Chemical science · 2022Article
- Mitigating long queues and waiting times with service resetting.PNAS nexus · 2022Article
- Sensing Enzyme Activation Heat Capacity at the Single-Molecule Level Using Gold-Nanorod-Based Optical Whispering Gallery Modes.ACS applied nano materials · 2021Article
- Effect of the PHY Domain on the Photoisomerization Step of the Forward PChemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Mechanical Unfolding of Single Polyubiquitin Molecules Reveals Evidence of Dynamic Disorder.ACS omega · 2020Article
- Single electron transfer events and dynamical heterogeneity in the small protein azurin fromChemical science · 2019Article
- Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition.Scientific reports · 2019Article
- Plasmon-Enhanced Single-Molecule Enzymology.ACS photonics · 2018Article
- Single-molecule theory of enzymatic inhibition.Nature communications · 2018Article
- Slow domain reconfiguration causes power-law kinetics in a two-state enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Monitoring the Activity of Immobilized Lipase with Quinizarin Diester Fluoro-Chromogenic Probe.Molecules (Basel, Switzerland) · 2017Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule techniques offer a unique tool for studying the dynamical behavior of individual molecules and provide the possibility to construct distributions from individual events rather than from a signal stemming from an ensemble of molecules. In biological systems, known for their complexity, these techniques make it possible to gain insights into the detailed spectrum of molecular conformational changes and activities. Here, we report on the direct observation of a single lipase-catalyzed reaction for extended periods of time (hours), by using confocal fluorescence microscopy. When adding a profluorescent substrate, the monitored enzymatic activity appears as a trajectory of "on-state" and "off-state" events. The waiting time probability density function of the off state and the state-correlation function fit stretched exponentials, independent of the substrate concentration in a certain range. The data analysis unravels oscillations in the logarithmic derivative of the off-state waiting time probability density function and correlations between off-state events. These findings imply that the fluctuating enzyme model, which involves a spectrum of enzymatic conformations that interconvert on the time scale of the catalytic activity, best describes the observed enzymatic activity. Based on this model, values for the coupling and reaction rates are extracted.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.