ArticleGenetics2005
Mitochondrial translation: elongation factor tu is essential in fission yeast and depends on an exchange factor conserved in humans but not in budding yeast.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Candida albicans PPR proteins are required for the expression of respiratory Complex I subunits.Genetics · 2024Article
- Designing phage cocktails to combat the emergence of bacteriophage-resistant mutants in multidrug-resistantMicrobiology spectrum · 2024Article
- In fission yeast, 65 non-essential mitochondrial proteins related to respiration and stress become essential in low-glucose conditions.Royal Society open science · 2023Article
- Translation in Mitochondrial Ribosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Translational activators and mitoribosomal isoforms cooperate to mediate mRNA-specific translation in Schizosaccharomyces pombe mitochondria.Nucleic acids research · 2021Article
- Multiple nutritional phenotypes of fission yeast mutants defective in genes encoding essential mitochondrial proteins.Open biology · 2021Article
- Atg43 tethers isolation membranes to mitochondria to promote starvation-induced mitophagy in fission yeast.eLife · 2020Article
- Systematic analysis of nuclear gene function in respiratory growth and expression of the mitochondrial genome inMicrobial cell (Graz, Austria) · 2020Article
- Down-regulation of TUFM impairs host cell interaction and virulence by Paracoccidioides brasiliensis.Scientific reports · 2019Article
- Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.G3 (Bethesda, Md.) · 2016Article
- Aim-less translation: loss of Saccharomyces cerevisiae mitochondrial translation initiation factor mIF3/Aim23 leads to unbalanced protein synthesis.Scientific reports · 2016Article
- Yeast model analysis of novel polymerase gamma variants found in patients with autosomal recessive mitochondrial disease.Human genetics · 2015Article
- Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.Mitochondrion · 2013Article
- Protein biosynthesis in mitochondria.Biochemistry. Biokhimiia · 2013Review
- Schizosaccharomyces pombe homologs of the Saccharomyces cerevisiae mitochondrial proteins Cbp6 and Mss51 function at a post-translational step of respiratory complex biogenesis.Mitochondrion · 2012Article
- A genome wide study in fission yeast reveals nine PPR proteins that regulate mitochondrial gene expression.Nucleic acids research · 2011Article
- Overexpression of TUF1 restores respiratory growth and fluconazole sensitivity to a Cryptococcus neoformans vad1Delta mutant.Microbiology (Reading, England) · 2010Article
- Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.Nature biotechnology · 2010Article
- Article
- Tdp1 protects against oxidative DNA damage in non-dividing fission yeast.The EMBO journal · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translation elongation factor EF-Tu is a GTPase that delivers amino-acylated tRNAs to the ribosome during the elongation step of translation. EF-Tu/GDP is recycled by the guanine nucleotide exchange factor EF-Ts. Whereas EF-Ts is lacking in S. cerevisiae, both translation factors are found in S. pombe and H. sapiens mitochondria, consistent with the known similarity between fission yeast and human cell mitochondrial physiology. We constructed yeast mutants lacking these elongation factors. We show that mitochondrial translation is vital for S. pombe, as it is for human cells. In a genetic background allowing the loss of mitochondrial functions, a block in mitochondrial translation in S. pombe leads to a major depletion of mtDNA. The relationships between EF-Ts and EF-Tu from both yeasts and humans were investigated through functional complementation and coexpression experiments and by a search for suppressors of the absence of the S. pombe EF-Ts. We find that S. cerevisiae EF-Tu is functionally equivalent to the S. pombe EF-Tu/EF-Ts couple. Point mutations in the S. pombe EF-Tu can render it independent of its exchange factor, thereby mimicking the situation in S. cerevisiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.