ArticleThe Journal of physiology2005
Tonic release of glutamate by a DIDS-sensitive mechanism in rat hippocampal slices.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 167 citations in OpenAlex.
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- The protective effects of statins in traumatic brain injury.Pharmacological reports : PR · 2024Review
- Glutamate-releasing BEST1 channel is a new target for neuroprotection against ischemic stroke with wide time window.Acta pharmaceutica Sinica. B · 2023Article
- Changes in glutamate and glutamine distributions in the retinas of cystine/glutamate antiporter knockout mice.Molecular vision · 2023Article
- GABAergic Regulation of Astroglial Gliotransmission through Cx43 Hemichannels.International journal of molecular sciences · 2022Article
- Honey, I shrunk the extracellular space: Measurements and mechanisms of astrocyte swelling.Glia · 2022Review
- Astrocytic Glutamatergic Transmission and Its Implications in Neurodegenerative Disorders.Cells · 2022Review
- Synaptic NMDA receptor activity at resting membrane potentials.Frontiers in cellular neuroscience · 2022Article
- Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.Pharmacological reviews · 2021Review
- Influence of glutamate and GABA transport on brain excitatory/inhibitory balance.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Glial Chloride Channels in the Function of the Nervous System Across Species.Advances in experimental medicine and biology · 2021Article
- The Paradox of Astroglial CaFrontiers in cellular neuroscience · 2020Review
- Glutamate-Releasing SWELL1 Channel in Astrocytes Modulates Synaptic Transmission and Promotes Brain Damage in Stroke.Neuron · 2019Article
- Higher ambient synaptic glutamate at inhibitory versus excitatory neurons differentially impacts NMDA receptor activity.Nature communications · 2018Article
- Neuronal Glutamate Transporters Control Dopaminergic Signaling and Compulsive Behaviors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Astrocyte-Mediated Neuronal Synchronization Properties Revealed by False Gliotransmitter Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Neuroprotective astrocyte-derived insulin/insulin-like growth factor 1 stimulates endocytic processing and extracellular release of neuron-bound Aβ oligomers.Molecular biology of the cell · 2017Article
- An autocrine purinergic signaling controls astrocyte-induced neuronal excitation.Scientific reports · 2017Article
- Turning down the volume: Astrocyte volume change in the generation and termination of epileptic seizures.Neurobiology of disease · 2017Review
19 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tonic release of glutamate into the extracellular space of the hippocampus and striatum is non-vesicular, and has been attributed largely to a cystine-glutamate exchanger which is blockable by the glutamate analogue (S)-4-carboxyphenylglycine (CPG). Tonic glutamate release may be functionally important: modulation of this release in the striatum has been suggested to underlie relapse in the use of cocaine. We monitored tonic glutamate release in area CA1 of hippocampal slices by measuring the glutamate receptor-mediated current evoked in pyramidal cells on block of Na(+)-dependent glutamate uptake with dl-threo-beta-benzyloxyaspartate (TBOA). Superfused cystine increased tonic glutamate release, and this increase was blocked by CPG, but CPG did not affect tonic glutamate release in the absence of superfused cystine. Tonic glutamate release was not affected by blocking gap junctional hemichannels with 18alpha-glycyrrhetinic acid, blocking ATP receptors with pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), blocking Ca(2)(+)-dependent exocytosis from neurones with Cd(2)(+) or bafilomycin, blocking Ca(2)(+)-dependent release from glia with indomethacin, or blocking anion channels with 5-nitro-2-(3-phenylpropyl amino) benzoic acid (NPPB) or tamoxifen. However tonic glutamate release was reduced by 4,4'-diisothiocyanostilbene-2,2'-disulphonic acid (DIDS), and was potentiated by inhibiting astrocytic conversion of glutamate to glutamine with methionine sulfoximine. These data suggest that although cystine-glutamate exchange is present in the hippocampus it does not generate significant tonic release of glutamate when the extracellular [cystine] is at a physiological level, and that tonic glutamate release is at least partly from astrocytes and is mediated by a DIDS-sensitive mechanism. Theoretical calculations suggest that a significant fraction of tonic glutamate release in hippocampal slices could occur via diffusion of glutamate across lipid membranes.
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