ArticleJournal of the American Society for Mass Spectrometry2005
Ultrafiltration tandem mass spectrometry of estrogens for characterization of structure and affinity for human estrogen receptors.
Article in Journal of the American Society for Mass Spectrometry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 77 citations in OpenAlex.
- Cannabinoids Block Cellular Entry of SARS-CoV-2 and the Emerging Variants.Journal of natural products · 2022Article
- Drug discovery from natural products using affinity selection-mass spectrometry.Drug discovery today. Technologies · 2021Review
- Selective Recognition of a Single HIV-1 G-Quadruplex by Ultrafast Small-Molecule Screening.Analytical chemistry · 2021Article
- Exploring new targets and chemical space with affinity selection-mass spectrometry.Nature reviews. Chemistry · 2021Review
- Drug Discovery on Natural Products: From Ion Channels to nAChRs, from Nature to Libraries, from Analytics to Assays.SLAS discovery : advancing life sciences R & D · 2019Review
- Development of a Magnetic Microbead Affinity Selection Screen (MagMASS) Using Mass Spectrometry for Ligands to the Retinoid X Receptor-α.Journal of the American Society for Mass Spectrometry · 2017Article
- Magnetic Microbead Affinity Selection Screening (MagMASS) of Botanical Extracts for Inhibitors of 15-Lipoxygenase.Journal of natural products · 2016Article
- Celecoxib influences steroid sulfonation catalyzed by human recombinant sulfotransferase 2A1.The Journal of steroid biochemistry and molecular biology · 2015Article
- Identification of Eupatilin from Artemisia argyi as a Selective PPARα Agonist Using Affinity Selection Ultrafiltration LC-MS.Molecules (Basel, Switzerland) · 2015Article
- Duo-molecularly imprinted polymer-coated magnetic particles for class-selective removal of endocrine-disrupting compounds from aqueous environment.Environmental science and pollution research international · 2013Article
- Evaluation of estrogenic activity of licorice species in comparison with hops used in botanicals for menopausal symptoms.PloS one · 2013Article
- Recent developments in protein-ligand affinity mass spectrometry.Analytical and bioanalytical chemistry · 2011Review
- Screening natural products for inhibitors of quinone reductase-2 using ultrafiltration LC-MS.Analytical chemistry · 2011Article
- Cyclooxygenase-2 inhibitors in ginger (Zingiber officinale).Fitoterapia · 2011Article
- Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays.Analytical and bioanalytical chemistry · 2010Article
- Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation.Pharmacological research · 2010Article
- Characterization of aromatase binding agents from the dichloromethane extract of Corydalis yanhusuo using ultrafiltration and liquid chromatography tandem mass spectrometry.Molecules (Basel, Switzerland) · 2010Article
- Screening and structural characterization of alpha-glucosidase inhibitors from hawthorn leaf flavonoids extract by ultrafiltration LC-DAD-MS(n) and SORI-CID FTICR MS.Journal of the American Society for Mass Spectrometry · 2009Article
- Research highlights from the UIC/NIH Center for Botanical Dietary Supplements Research for Women's Health: Black cohosh from the field to the clinic.Pharmaceutical biology · 2009Article
- Technologies and experimental approaches at the National Institutes of Health Botanical Research Centers.The American journal of clinical nutrition · 2008Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Although hormone replacement therapy (HRT) is used by post-menopausal women for the relief of menopausal symptoms and the potential reduction of osteoporosis, HRT also increases their risk of Alzheimer's disease, stroke, breast cancer, and endometrial cancer. Since the majority of these effects are associated primarily with estrogen binding to only one of the estrogen receptors (ER), new assays are needed that can more efficiently evaluate ER-binding and identify ligands selective for ER-alpha and ER-beta. High performance liquid chromatography-tandem mass spectrometry (LC-MS-MS) was combined with ultrafiltration as a new method to investigate the relative binding of compounds to the ERs and to evaluate the structures of these estrogens. Mixtures of estradiol and six equine estrogens, including equilin, equilenin, 8,9-dehydroestrone, and their 17beta-hydroxyl derivatives, were assayed simultaneously to determine their relative binding to human ER-alpha and ER-beta. Estrogens containing a 17beta-OH group were found to have higher relative affinities for the estrogen receptors than their ketone analogs. In addition, 17beta-EN showed selectivity for binding to ER-beta over ER-alpha. The results were compared to the IC50 values obtained by using a conventional radiolabled estradiol competitive binding assay. Finally, the utility of negative ion electrospray tandem mass spectrometry for the unambiguous identification of these estrogen isomers was investigated. Several characteristic recyclization pathways during tandem mass spectrometry were identified, which might be useful for distinguishing related estrogens.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.