Evidence map›Paper›PMID 15694003›Full record

ArticleBMC genomics2005

Development of a cDNA array for chicken gene expression analysis.

Joan Burnside, Paul Neiman, Jianshan Tang, Ryan Basom, Richard Talbot, Mark Aronszajn, David Burt, Jeff Delrow

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
8.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 76 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Joan BurnsideDelaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Room 229, Newark, DE 19711, USA. joan@udel.edu
Paul Neiman
Jianshan Tang
Ryan Basom
Richard Talbot
Mark Aronszajn
David Burt
Jeff Delrow
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa · ZAFred Hutch Cancer Center · USRoslin Institute · GBGlaxoSmithKline (United States) · USUniversity of Delaware · US

Funding

MOLECULAR MECHANISMS IN NEOPLASIAR01CA020068 · NCI · FRED HUTCHINSON CANCER RESEARCH CENTER · PI NEIMAN, PAUL ERIC · 1985 to 2008
$2.6M
NCI NIH HHS R01 CA020068NCI NIH HHS R01 CA20068
6 · The paper itself

Abstract

backgroundThe application of microarray technology to functional genomic analysis in the chicken has been limited by the lack of arrays containing large numbers of genes.

resultsWe have produced cDNA arrays using chicken EST collections generated by BBSRC, University of Delaware and the Fred Hutchinson Cancer Research Center. From a total of 363,838 chicken ESTs representing 24 different adult or embryonic tissues, a set of 11,447 non-redundant ESTs were selected and added to an existing collection of clones (4,162) from immune tissues and a chicken bursal cell line (DT40). Quality control analysis indicates there are 13,007 useable features on the array, including 160 control spots. The array provides broad coverage of mRNAs expressed in many tissues; in addition, clones with expression unique to various tissues can be detected.

conclusionsA chicken multi-tissue cDNA microarray with 13,007 features is now available to academic researchers from http://genomics@fhcrc.org. Sequence information for all features on the array is in GenBank, and clones can be readily obtained. Targeted users include researchers in comparative and developmental biology, immunology, vaccine and agricultural technology. These arrays will be an important resource for the entire research community using the chicken as a model.

Indexed as

Gene Expression RegulationAnimalsBrainCell LineChickensCloning, MolecularCluster AnalysisComputational BiologyDatabases, GeneticDNA, ComplementaryExpressed Sequence TagsFibroblastsGene ExpressionGene Expression ProfilingGene LibraryGenomicsDNA, ComplementaryRNA, Messenger

Identifiers

PMID15694003
PMCPMC549535
OpenAlexW1847570260

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.