ArticleThe Journal of experimental medicine1992
Molecular characterization of a locus required for hyaluronic acid capsule production in group A streptococci.
Article in The Journal of experimental medicine, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- Global spread of Streptococcus pyogenes A genomics-supported narrative review.FEMS microbiology reviews · 2025Review
- Review
- Detoxification of reactive oxygen species by the hyaluronic acid capsule of group AInfection and immunity · 2023Article
- The Streptococcus pyogenes hyaluronic acid capsule promotes experimental nasal and skin infection by preventing neutrophil-mediated clearance.PLoS pathogens · 2022Article
- Genetics of Group A Streptococci.Microbiology spectrum · 2019Review
- Capsular Polysaccharide of Group AMicrobiology spectrum · 2019Article
- Mutual exclusivity of hyaluronan and hyaluronidase in invasive group A Streptococcus.The Journal of biological chemistry · 2014Article
- Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules.FEMS microbiology reviews · 2014Review
- Disease manifestations and pathogenic mechanisms of Group A Streptococcus.Clinical microbiology reviews · 2014Review
- A conserved UDP-glucose dehydrogenase encoded outside the hasABC operon contributes to capsule biogenesis in group A Streptococcus.Journal of bacteriology · 2012Article
- Article
- Complete genome sequence and immunoproteomic analyses of the bacterial fish pathogen Streptococcus parauberis.Journal of bacteriology · 2011Article
- Two capsular polysaccharides enable Bacillus cereus G9241 to cause anthrax-like disease.Molecular microbiology · 2011Article
- Rgg influences the expression of multiple regulatory loci to coregulate virulence factor expression in Streptococcus pyogenes.Infection and immunity · 2002Article
- Pathogenesis of group A streptococcal infections.Clinical microbiology reviews · 2000Review
- A two-component regulatory system, CsrR-CsrS, represses expression of three Streptococcus pyogenes virulence factors, hyaluronic acid capsule, streptolysin S, and pyrogenic exotoxin B.Infection and immunity · 1999Article
- A response regulator that represses transcription of several virulence operons in the group A streptococcus.Journal of bacteriology · 1999Article
- Characterization of the galU gene of Streptococcus pneumoniae encoding a uridine diphosphoglucose pyrophosphorylase: a gene essential for capsular polysaccharide biosynthesis.The Journal of experimental medicine · 1998Article
- Reduced virulence of group A streptococcal Tn916 mutants that do not produce streptolysin S.Infection and immunity · 1998Article
- Culture Conditions Affect the Molecular Weight Properties of Hyaluronic Acid Produced by Streptococcus zooepidemicus.Applied and environmental microbiology · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To characterize the production of hyaluronate capsule by the membrane-associated enzyme hyaluronate synthase (HAS), group A streptococci from a recent outbreak of acute rheumatic fever were mutagenized via Tn916 insertion. Acapsular transconjugants harboring multiple, nontandem copies of the transposon were identified and found to lack HAS activity (less than 1% of wild-type levels). Generalized transduction was then performed to determine which Tn916 insertion was responsible for the HAS- phenotype. These marker exchange experiments resulted in the isolation of two distinct classes of acapsular transductants, designated WF61 and WF62. Both transductants also lacked significant HAS activity, and excision of the transposon from WF62 restored capsular hyaluronate production. Southern analysis of WF61 DNA demonstrated a large deletion of genomic DNA adjacent to the Tn916 insertion. This deletion event is presumably responsible for the observed stability of the acapsular phenotype of WF61. Further analyses of transductant whole-cell DNA indicated that the transposon insertions of WF61 and WF62 were separated by 2.5 kb. These studies define a locus required for hyaluronate capsule production in group A streptococci. Further genetic analysis of this locus has identified a gene required for HAS activity which wasd inactivated by TN916 in WF62 and deleted in WF61.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.