ArticleThe EMBO journal2005
Processing of DNA for nonhomologous end-joining by cell-free extract.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed.
- Review
- Pathogenic variants in human DNA damage repair genes mostly arose after the latest human out-of-Africa migration.Frontiers in genetics · 2024Article
- Review
- Chromosomal radiosensitivity of human breast carcinoma cells and blood lymphocytes following photon and proton exposures.Radiation and environmental biophysics · 2023Article
- Characterizing the Repair of DNA Double-Strand Breaks: A Review of Surrogate Plasmid-Based Reporter Methods.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Increased mutation efficiency of CRISPR/Cas9 genome editing in banana by optimized construct.PeerJ · 2022Article
- Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration.The CRISPR journal · 2021Article
- Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience.Plants (Basel, Switzerland) · 2021Review
- Repair of DNA Double-Strand Breaks by the Nonhomologous End Joining Pathway.Annual review of biochemistry · 2021Review
- Investigating Molecular Determinants of Cancer Cell Resistance to Ionizing Radiation Through an Integrative Bioinformatics Approach.Frontiers in cell and developmental biology · 2021Article
- A Mechanism to Minimize Errors during Non-homologous End Joining.Molecular cell · 2020Article
- Application and future perspective of CRISPR/Cas9 genome editing in fruit crops.Journal of integrative plant biology · 2020Review
- PAXX and its paralogs synergistically direct DNA polymerase λ activity in DNA repair.Nature communications · 2018Article
- Senoptosis: non-lethal DNA cleavage as a route to deep senescence.Oncotarget · 2017Article
- Bridging of double-stranded breaks by the nonhomologous end-joining ligation complex is modulated by DNA end chemistry.Nucleic acids research · 2017Article
- Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida.Scientific reports · 2016Article
- Cas9-catalyzed DNA Cleavage Generates Staggered Ends: Evidence from Molecular Dynamics Simulations.Scientific reports · 2016Article
- Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining.Cell reports · 2016Article
- Mechanistic Modelling and Bayesian Inference Elucidates the Variable Dynamics of Double-Strand Break Repair.PLoS computational biology · 2016Article
- Opposing roles of RNF8/RNF168 and deubiquitinating enzymes in ubiquitination-dependent DNA double-strand break response signaling and DNA-repair pathway choice.Journal of radiation research · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
In mammalian cells, nonhomologous end-joining (NHEJ) repairs DNA double-strand breaks created by ionizing radiation and V(D)J recombination. We have developed a cell-free system capable of processing and joining noncompatible DNA ends. The system had key features of NHEJ in vivo, including dependence on Ku, DNA-PKcs, and XRCC4/Ligase4. The NHEJ reaction had striking properties. Processing of noncompatible ends involved polymerase and nuclease activities that often stabilized the alignment of opposing ends by base pairing. To achieve this, polymerase activity efficiently synthesized DNA across discontinuities in the template strand, and nuclease activity removed a limited number of nucleotides back to regions of microhomology. Processing was suppressed for DNA ends that could be ligated directly, biasing the reaction to preserve DNA sequence and maintain genomic integrity. DNA sequence internal to the ends influenced the spectrum of processing events for noncompatible ends. Furthermore, internal DNA sequence strongly influenced joining efficiency, even in the absence of processing. These results support a model in which DNA-PKcs plays a central role in regulating the processing of ends for NHEJ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.