ArticleApplied and environmental microbiology2005
Unique kinetic properties of phenol-degrading variovorax strains responsible for efficient trichloroethylene degradation in a chemostat enrichment culture.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 81 citations in OpenAlex.
- Control of alluvial aquifer architecture on reductive and oxidative dechlorination of chloroethenes.Environmental science and pollution research international · 2026Article
- Stable coexistence and phenol-degradation expression of Cupriavidus sp. strain P-10 and Comamonas thiooxydans strain R2 in phenol-competitive chemostat culture.Applied microbiology and biotechnology · 2025Article
- Stable States of a Microbial Community Are Formed by Dynamic Metabolic Networks with Members Functioning to Achieve Both Robustness and Plasticity.Microbes and environments · 2024Article
- Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system.Frontiers in microbiology · 2023Article
- Characteristics and Comparative Genomic Analysis of a Novel Virus, VarioGold, the First Bacteriophage ofInternational journal of molecular sciences · 2022Article
- Recent advances and trends of trichloroethylene biodegradation: A critical review.Frontiers in microbiology · 2022Review
- Imbalance in Carbon and Nitrogen Metabolism in Comamonas testosteroni R2 Is Caused by Negative Feedback and Rescued by L-arginine.Microbes and environments · 2021Article
- Draft Genome Sequence of Phenol-Degrading Variovorax boronicumulans Strain c24.Microbiology resource announcements · 2020Article
- Isoprene Oxidation by the Gram-Negative Model bacteriumMicroorganisms · 2020Article
- Microbial degradation of chloroethenes: a review.Environmental science and pollution research international · 2017Review
- Microbial Degradation of Polyhydroxyalkanoates with Different Chemical Compositions and Their Biodegradability.Microbial ecology · 2017Article
- Draft Genome Sequence of Pseudomonas sp. LAB-08 Isolated from Trichloroethene-Contaminated Aquifer Soil.Genome announcements · 2016Article
- Reductive dechlorination in recalcitrant sources of chloroethenes in the transition zone between aquifers and aquitards.Environmental science and pollution research international · 2016Article
- Interspecies interactions are an integral determinant of microbial community dynamics.Frontiers in microbiology · 2015Article
- Challenges for complex microbial ecosystems: combination of experimental approaches with mathematical modeling.Microbes and environments · 2013Review
- Properties of phenol-removal aerobic granules during normal operation and shock loading.Journal of industrial microbiology & biotechnology · 2010Article
- Genetic diversity and host range of rhizobia nodulating Lotus tenuis in typical soils of the Salado River Basin (Argentina).Applied and environmental microbiology · 2009Article
- Effect of 2,4,6-trinitrotoluene on soil bacterial communities.Journal of industrial microbiology & biotechnology · 2008Article
- Mutation of Candida tropicalis by irradiation with a He-Ne laser to increase its ability to degrade phenol.Applied and environmental microbiology · 2007Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A chemostat enrichment of soil bacteria growing on phenol as the sole carbon source has been shown to exhibit quite high trichloroethylene (TCE)-degrading activities. To identify the bacterial populations responsible for the high TCE-degrading activity, a multidisciplinary survey of the chemostat enrichment was conducted by employing molecular-ecological and culture-dependent approaches. Three chemostat enrichment cultures were newly developed under different phenol-loading conditions (0.25, 0.75, and 1.25 g liter(-1) day(-1)) in this study, and the TCE-degrading activities of the enrichments were measured. Among them, the enrichment at 0.75 g liter(-1) day(-1) (enrichment 0.75) expressed the highest activity. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments detected a Variovorax ribotype as the strongest band in enrichment 0.75; however, it was not a major ribotype in the other samples. Bacteria were isolated from enrichment 0.75 by direct plating, and their 16S rRNA genes and genes encoding the largest subunit of phenol hydroxylase (LmPHs) were analyzed. Among the bacteria isolated, several strains were affiliated with the genus Variovorax and were shown to have high-affinity-type LmPHs. The LmPH of the Variovorax strains was also detected as the major genotype in enrichment 0.75. Kinetic analyses of phenol and TCE degradation revealed, however, that these strains exhibited quite low affinity for phenol compared to other phenol-degrading bacteria, while they showed quite high specific TCE-degrading activities and relatively high affinity for TCE. Owing to these unique kinetic traits, the Variovorax strains can obviate competitive inhibition of TCE degradation by the primary substrate of the catabolic enzyme (i.e., phenol), contributing to the high TCE-degrading activity of the chemostat enrichments. On the basis of physiological information, mechanisms accounting for the way the Variovorax population overgrew the chemostat enrichment are discussed.
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