ArticleApplied and environmental microbiology2005
Tetracycline-dependent conditional gene knockout in Bacillus subtilis.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Rewiring Carbon Metabolism in Bacillus methanolicus via Heterologous Phosphoketolase Expression Enhances Biomass Yield From Methanol and Reduces COMicrobial biotechnology · 2026Article
- Tet-ON and Tet-OFF regulation in hypervirulentMicrobiology spectrum · 2026Article
- A multi-tetracycline responsive induction system for gene expression inApplied and environmental microbiology · 2026Article
- A Multi-Tetracycline Responsive Induction System for Gene Expression inbioRxiv : the preprint server for biology · 2025Article
- Status quo of tet regulation in bacteria.Microbial biotechnology · 2022Review
- An operator-based expression toolkit forProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Expansion of the tetracycline-dependent regulation toolbox for Helicobacter pylori.Applied and environmental microbiology · 2015Article
- Recombinant reporter assay using transcriptional machinery of Mycobacterium tuberculosis.Journal of bacteriology · 2015Article
- Current development in genetic engineering strategies of Bacillus species.Microbial cell factories · 2014Review
- New vectors for chromosomal integration enable high-level constitutive or inducible magnetosome expression of fusion proteins in Magnetospirillum gryphiswaldense.Applied and environmental microbiology · 2014Article
- Half-life measurements of chemical inducers for recombinant gene expression.Journal of biological engineering · 2014Article
- Development of a tetracycline-inducible gene expression system for the study of Helicobacter pylori pathogenesis.Applied and environmental microbiology · 2013Article
- High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.BMC genomics · 2012Article
- Analysis and application of Bacillus subtilis sortases to anchor recombinant proteins on the cell wall.AMB Express · 2011Article
- Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis.Journal of bacteriology · 2011Article
- Staphylococcus aureus TargetArray: comprehensive differential essential gene expression as a mechanistic tool to profile antibacterials.Antimicrobial agents and chemotherapy · 2010Article
- Recombinant Mycobacterium bovis BCG as an HIV vaccine vector.Current HIV research · 2010Review
- New architectures for Tet-on and Tet-off regulation in Staphylococcus aureus.Applied and environmental microbiology · 2010Article
- Improved tetracycline repressors for gene silencing in mycobacteria.Nucleic acids research · 2009Article
- Codon-optimized fluorescent proteins designed for expression in low-GC gram-positive bacteria.Applied and environmental microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reversible tetracycline-dependent gene regulation allows induction of expression with the tetracycline repressor (TetR) or gene silencing with the newly developed reverse mutant revTetR. We report here the implementation of both approaches with full regulatory range in gram-positive bacteria as exemplified in Bacillus subtilis. A chromosomally located gene is controlled by one or two tet operators. The precise adjustment of regulatory windows is accomplished by adjusting tetR or revtetR expression via different promoters. The most efficient induction was 300-fold in the presence of 0.4 microM anhydrotetracycline obtained with a Pr-xylA-tetR fusion. Reversible 500-fold gene knockouts were obtained in B. subtilis after adjusting expression of revTetR by synthetically designed promoters. We anticipate that these tools will also be useful in many other gram-positive bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.