ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
A cytosolic residue mediates Mg2+ block and regulates inward current amplitude of a transient receptor potential channel.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 78 citations in OpenAlex.
- (-)-Englerin A binding to human TRPC5 exposes an aromatic interaction network in channel activation.Nature communications · 2026Article
- Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel.Nature structural & molecular biology · 2025Article
- Article
- Functional determinants of lysophospholipid- and voltage-dependent regulation of TRPC5 channel.Cellular and molecular life sciences : CMLS · 2024Article
- Direct modulation of TRPC ion channels by Gα proteins.Frontiers in physiology · 2024Review
- Molecular architecture of the GαNature communications · 2023Article
- Article
- Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current.International journal of molecular sciences · 2021Article
- Voltage-dependent modulation of TRPA1 currents by diphenhydramine.Cell calcium · 2020Article
- Review
- Analysis of interaction between intracellular spermine and transient receptor potential canonical 4 channel: multiple candidate sites of negatively charged amino acids for the inward rectification of transient receptor potential canonical 4.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2020Article
- Review
- Cryo-EM structure of TRPC5 at 2.8-Å resolution reveals unique and conserved structural elements essential for channel function.Science advances · 2019Article
- Treasure troves of pharmacological tools to study transient receptor potential canonical 1/4/5 channels.British journal of pharmacology · 2019Review
- Structure of the mouse TRPC4 ion channel.Nature communications · 2018Article
- Smooth Muscle Ion Channels and Regulation of Vascular Tone in Resistance Arteries and Arterioles.Comprehensive Physiology · 2017Review
- Molecular Determinants of the Sensitivity to Gq/11-Phospholipase C-dependent Gating, Gd3+ Potentiation, and Ca2+ Permeability in the Transient Receptor Potential Canonical Type 5 (TRPC5) Channel.The Journal of biological chemistry · 2017Article
- Regulator of G-protein signalling and GoLoco proteins suppress TRPC4 channel function via acting at Gαi/o.The Biochemical journal · 2016Article
- Intracellular spermine blocks TRPC4 channel via electrostatic interaction with C-terminal negative amino acids.Pflugers Archiv : European journal of physiology · 2016Article
- Permeation, regulation and control of expression of TRP channels by trace metal ions.Pflugers Archiv : European journal of physiology · 2015Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of the transient receptor potential (TRP) cation channel family control a wide variety of cellular functions by regulating calcium influx. In neurons, TRP channels may also modulate cell excitability. TRPC5 is a neuronal TRP channel that plays a role in controlling neurite extension in the hippocampus. Transiently expressed TRPC5 exhibits a doubly rectifying current-voltage relationship characterized by relatively large inward currents and a unique outwardly rectifying current with a "flat" segment between +10 and +40 mV that may be attributable to Mg2+ block. We find that intracellular Mg2+ blocks the outward current through TRPC5 with an IC50 of 457 microM. The block is mediated by a cytosolic aspartate residue, D633, situated between the termination of the sixth transmembrane domain and the "TRP box." The substitution of noncharged or positively charged residues for the negatively charged D633 resulted in a channel with markedly reduced inward currents, in addition to decreased Mg2+ block. This suggests that electrostatic attraction of cations by D633 may contribute to inward current amplitude in TRPC5. We propose that cytosolic negatively charged residues can modulate the conductivity of TRP cation channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.