ArticleCurrent genetics2005
Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum.
Article in Current genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.AMB Express · 2020Article
- The Gibberellin ProducerFrontiers in bioengineering and biotechnology · 2020Review
- Yeast two-hybrid screening reveals a dual function for the histone acetyltransferase GcnE by controlling glutamine synthesis and development in Aspergillus fumigatus.Current genetics · 2019Article
- Recurrent Neural Network for Predicting Transcription Factor Binding Sites.Scientific reports · 2018Article
- Friends or foes? Emerging insights from fungal interactions with plants.FEMS microbiology reviews · 2016Review
- Function of a p24 Heterodimer in Morphogenesis and Protein Transport in Penicillium oxalicum.Scientific reports · 2015Article
- A modular plasmid system for protein co-localization and bimolecular fluorescence complementation in filamentous fungi.Current genetics · 2014Article
- BEM46 shows eisosomal localization and association with tryptophan-derived auxin pathway in Neurospora crassa.Eukaryotic cell · 2014Article
- Members of the Penicillium chrysogenum velvet complex play functionally opposing roles in the regulation of penicillin biosynthesis and conidiation.Eukaryotic cell · 2013Article
- Role of Aspergillus niger acrA in arsenic resistance and its use as the basis for an arsenic biosensor.Applied and environmental microbiology · 2012Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- The Aspergillus nidulans MAPK module AnSte11-Ste50-Ste7-Fus3 controls development and secondary metabolism.PLoS genetics · 2012Article
- Concentration of antifungal agents within host cell membranes: a new paradigm governing the efficacy of prophylaxis.Antimicrobial agents and chemotherapy · 2011Article
- Rapid quantification of lipids in Acremonium chrysogenum using Oil red O.Current microbiology · 2011Article
- FfVel1 and FfLae1, components of a velvet-like complex in Fusarium fujikuroi, affect differentiation, secondary metabolism and virulence.Molecular microbiology · 2010Article
- Two components of a velvet-like complex control hyphal morphogenesis, conidiophore development, and penicillin biosynthesis in Penicillium chrysogenum.Eukaryotic cell · 2010Article
- The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes.Nucleic acids research · 2010Article
- Simultaneous visualization of two protein complexes in a single plant cell using multicolor fluorescence complementation analysis.Plant molecular biology · 2009Article
- MCM-GINS and MCM-MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast.BMC cell biology · 2009Article
- The nuclear migration protein NUDF/LIS1 forms a complex with NUDC and BNFA at spindle pole bodies.Eukaryotic cell · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using bimolecular fluorescence complementation assays, we were able to demonstrate protein-protein interaction of the transcription factors AcFKH1 and CPCR1 in living cells from the filamentous fungus Acremonium chrysogenum. This was accomplished by splitting the gene for the enhanced yellow fluorescent protein (EYFP) into two parts encoding the N- and C-terminus. Both fragments were fused to different gene derivatives of the fungal transcription factors. The recombinant plasmids were used to generate transgenic fungal strains for subsequent confocal laser microscopy. Only when the full-length transcription factors were fused to EYFP fragments yellow fluorescence was observed due to the bimolecular complementation of both chimeric proteins. The nuclear localization of the protein-protein interaction was verified by staining fungal cells with the nucleic acid dye TOTO-3.
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