ArticleNucleic acids research2005
In vivo RNA localization of I factor, a non-LTR retrotransposon, requires a cis-acting signal in ORF2 and ORF1 protein.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Review
- Transposable elements inMobile DNA · 2020Review
- Article
- L1 retrotransposon antisense RNA within ASAR lncRNAs controls chromosome-wide replication timing.The Journal of cell biology · 2018Article
- Retrotransposons Mimic Germ Plasm Determinants to Promote Transgenerational Inheritance.Current biology : CB · 2017Article
- Specific activation of an I-like element in Drosophila interspecific hybrids.Genome biology and evolution · 2014Article
- Mechanism by which a LINE protein recognizes its 3' tail RNA.Nucleic acids research · 2014Article
- RNA:RNA interaction can enhance RNA localization in Drosophila oocytes.RNA (New York, N.Y.) · 2012Article
- Independently derived targeting of 28S rDNA by A- and D-clade R2 retrotransposons: Plasticity of integration mechanism.Mobile genetic elements · 2011Article
- Non-long terminal repeat (non-LTR) retrotransposons: mechanisms, recent developments, and unanswered questions.Mobile DNA · 2010Article
- Nucleic-acid-binding properties of the C2-L1Tc nucleic acid chaperone encoded by L1Tc retrotransposon.The Biochemical journal · 2009Article
- A bioinformatics search pipeline, RNA2DSearch, identifies RNA localization elements in Drosophila retrotransposons.RNA (New York, N.Y.) · 2009Article
- Non-LTR retrotransposons encode noncanonical RRM domains in their first open reading frame.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- piRNA-mediated nuclear accumulation of retrotransposon transcripts in the Drosophila female germline.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Essential domains for ribonucleoprotein complex formation required for retrotransposition of telomere-specific non-long terminal repeat retrotransposon SART1.Molecular and cellular biology · 2006Article
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Authors and funding
6 authors.
Funding
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Abstract
According to the current model of non-LTR retrotransposon (NLR) mobilization, co-expression of the RNA transposition intermediate, and the proteins it encodes (ORF1p and ORF2p), is a requisite for the formation of cytoplasmic ribonucleoprotein complexes which contain necessary elements to complete a retrotransposition cycle later in the nucleus. To understand these early processes of NLR mobilization, here we analyzed in vivo the protein and RNA expression patterns of the I factor, a model NLR in Drosophila. We show that ORF1p and I factor RNA, specifically produced during transposition, are co-expressed and tightly co-localize with a specific pattern (Loc+) exclusively in the cytoplasm of germ cells permissive for retrotransposition. Using an ORF2 mutated I factor, we show that ORF2p plays no role in the Loc+ patterning. With deletion derivatives of an I factor we define an RNA localization signal required to display the Loc+ pattern. Finally, by complementation experiments we show that ORF1p is necessary for the efficient localization of I factor RNA. Our data suggest that ORF1p is involved in proper folding and stabilization of I factor RNA for efficient targeting, through Loc+ patterning, to the nuclear neighborhood where downstream steps of the retrotransposition process occur.
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