ArticleNucleic acids research2005
Licensing for DNA replication requires a strict sequential assembly of Cdc6 and Cdt1 onto chromatin in Xenopus egg extracts.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Who gets a license: DNA synthesis in quiescent cells re-entering the cell cycle.Current genetics · 2021Review
- Towards Better Understanding of KSHV Life Cycle: from Transcription and Posttranscriptional Regulations to Pathogenesis.Virologica Sinica · 2019Review
- Tsetse fly (Glossina pallidipes) midgut responses to Trypanosoma brucei challenge.Parasites & vectors · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing.Oncotarget · 2016Article
- KSHV Genome Replication and Maintenance.Frontiers in microbiology · 2016Review
- Adenovirus replaces mitotic checkpoint controls.Journal of virology · 2015Article
- YAP regulates S-phase entry in endothelial cells.PloS one · 2015Article
- Activation of a human chromosomal replication origin by protein tethering.Nucleic acids research · 2013Article
- Helicase loading at chromosomal origins of replication.Cold Spring Harbor perspectives in biology · 2013Review
- The p53-p21WAF1 checkpoint pathway plays a protective role in preventing DNA rereplication induced by abrogation of FOXF1 function.Cellular signalling · 2012Article
- How dormant origins promote complete genome replication.Trends in biochemical sciences · 2011Review
- Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication.Biochimica et biophysica acta · 2011Article
- Synergistic chemosensitivity of triple-negative breast cancer cell lines to poly(ADP-Ribose) polymerase inhibition, gemcitabine, and cisplatin.Cancer research · 2010Article
- Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex.Nucleic acids research · 2010Article
- Cell cycle G2/M arrest through an S phase-dependent mechanism by HIV-1 viral protein R.Retrovirology · 2010Article
- The minichromosome maintenance proteins 2-7 (MCM2-7) are necessary for RNA polymerase II (Pol II)-mediated transcription.The Journal of biological chemistry · 2009Article
- Repression of nascent strand elongation by deregulated Cdt1 during DNA replication in Xenopus egg extracts.Molecular biology of the cell · 2009Article
- Schizosaccharomyces pombe Noc3 is essential for ribosome biogenesis and cell division but not DNA replication.Eukaryotic cell · 2008Article
- Differential requirement of DNA replication factors for subtelomeric ARS consensus sequence protosilencers in Saccharomyces cerevisiae.Genetics · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Replication origins are licensed for a single initiation event by the loading of Mcm2-7 proteins during late mitosis and G1. Sequential associations of origin recognition complex, Cdc6 and Mcm2-7 are essential for completion of the licensing. Although Cdt1 also binds to the chromatin when the licensing reaction takes place, whether the binding is a requirement for Cdt1 to function is unclear. To analyze the relevance of the chromatin association of Cdt1, we carried out chromatin transfer experiments using either immunodepleted Xenopus egg extracts or purified proteins. Licensing assay and immunoblotting analyses indicated that Cdt1 could only license DNA replication and load Mcm2-7 onto DNA when it binds to chromatin that has already associated with Cdc6. These results provide evidence supporting that Cdc6 and Cdt1 must bind to chromatin in a strict order for DNA licensing to occur.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.