ArticleNucleic acids research2005
Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 58 citations in OpenAlex.
- Mouse resources at the RIKEN BioResource Research Center and the National BioResource Project core facility in Japan.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor.Genome research · 2021Article
- Mutant non-coding RNA resource in mouse embryonic stem cells.Disease models & mechanisms · 2021Article
- Applications ofStem cells international · 2021Review
- Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis.Nucleic acids research · 2018Article
- The number and distribution of AMPA receptor channels containing fast kinetic GluA3 and GluA4 subunits at auditory nerve synapses depend on the target cells.Brain structure & function · 2017Article
- Transposons As Tools for Functional Genomics in Vertebrate Models.Trends in genetics : TIG · 2017Review
- PiggyBac transposon-based polyadenylation-signal trap for genome-wide mutagenesis in mice.Scientific reports · 2016Article
- Forward Genetic Approach to Uncover Stress Resistance Genes in Mice - A High-throughput Screen in ES Cells.Journal of visualized experiments : JoVE · 2015Article
- Essential amino acid transporter Lat4 (Slc43a2) is required for mouse development.The Journal of physiology · 2015Article
- Screening for stress-resistance mutations in the mouse.Frontiers in genetics · 2014Article
- FLASH/casp8ap2 is indispensable for early embryogenesis but dispensable for proliferation and differentiation of ES cells.PloS one · 2014Article
- Evidence that the Upf1-related molecular motor scans the 3'-UTR to ensure mRNA integrity.Nucleic acids research · 2012Article
- Conditional gene manipulation: Cre-ating a new biological era.Journal of Zhejiang University. Science. B · 2012Review
- Mixture of differentially tagged Tol2 transposons accelerates conditional disruption of a broad spectrum of genes in mouse embryonic stem cells.Nucleic acids research · 2012Article
- Article
- Gene trap mutagenesis in the mouse.Methods in enzymology · 2010Article
- Efficient transposition of Tol2 in the mouse germline.Genetics · 2009Article
- Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells.Nucleic acids research · 2009Article
- Silencing and transcriptional properties of the imprinted Airn ncRNA are independent of the endogenous promoter.The EMBO journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An international collaborative project has been proposed to inactivate all mouse genes in embryonic stem (ES) cells using a combination of random and targeted insertional mutagenesis techniques. Random gene trapping will be the first choice in the initial phase, and gene-targeting experiments will then be carried out to individually knockout the remaining 'difficult-to-trap' genes. One of the most favored techniques of random insertional mutagenesis is promoter trapping, which only disrupts actively transcribed genes. Polyadenylation (poly-A) trapping, on the other hand, can capture a broader spectrum of genes including those not expressed in the target cells, but we noticed that it inevitably selects for the vector integration into the last introns of the trapped genes. Here, we present evidence that this remarkable skewing is caused by the degradation of a selectable-marker mRNA used for poly-A trapping via an mRNA-surveillance mechanism, nonsense-mediated mRNA decay (NMD). We also report the development of a novel poly-A-trap strategy, UPATrap, which suppresses NMD of the selectable-marker mRNA and permits the trapping of transcriptionally silent genes without a bias in the vector-integration site. We believe the UPATrap technology enables a simple and straightforward approach to the unbiased inactivation of all mouse genes in ES cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.