ArticleJournal of bacteriology2005
GlnD is essential for NifA activation, NtrB/NtrC-regulated gene expression, and posttranslational regulation of nitrogenase activity in the photosynthetic, nitrogen-fixing bacterium Rhodospirillum rubrum.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Function, Structure, and Regulation of Nitrogen Fixation-like Metalloproteins for Nitrogen, Energy, Carbon, and Sulfur Metabolism.Chemical reviews · 2026Review
- The influence of co-encapsulatedFood chemistry: X · 2024Article
- Unveiling Agricultural Biotechnological Prospects: The Draft Genome Sequence of Stenotrophomonas geniculata LGMB417.Current microbiology · 2024Article
- Nitric oxide sensor NsrR is the key direct regulator of magnetosome formation and nitrogen metabolism in Magnetospirillum.Nucleic acids research · 2024Article
- A singular PpaA/AerR-like protein inmSystems · 2023Article
- Aerobic-anaerobic transition boosts poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in Rhodospirillum rubrum: the key role of carbon dioxide.Microbial cell factories · 2023Article
- Influence of host genotype in establishing root associated microbiome ofFrontiers in microbiology · 2022Article
- Deciphering the potential of a plant growth promoting endophyteFrontiers in microbiology · 2022Article
- Genome-Wide Analyses of Proteome and Acetylome inFrontiers in microbiology · 2021Article
- Extending Viability ofFrontiers in microbiology · 2019Article
- Energy shifts induce membrane sequestration of DraG in Rhodospirillum rubrum independent of the ammonium transporters and diazotrophic conditions.FEMS microbiology letters · 2018Article
- Sodium Lactate Negatively Regulates Shewanella putrefaciens CN32 Biofilm Formation via a Three-Component Regulatory System (LrbS-LrbA-LrbR).Applied and environmental microbiology · 2017Article
- Ammonia-Oligotrophic and Diazotrophic Heavy Metal-Resistant Serratia liquefaciens Strains from Pioneer Plants and Mine Tailings.Microbial ecology · 2016Article
- Iron response regulator protein IrrB in Magnetospirillum gryphiswaldense MSR-1 helps control the iron/oxygen balance, oxidative stress tolerance, and magnetosome formation.Applied and environmental microbiology · 2015Article
- Metabolic analysis of Chlorobium chlorochromatii CaD3 reveals clues of the symbiosis in 'Chlorochromatium aggregatum'.The ISME journal · 2014Article
- Identification of a new gene required for the biosynthesis of rhodoquinone in Rhodospirillum rubrum.Journal of bacteriology · 2012Article
- Crystal structures of the apo and ATP bound Mycobacterium tuberculosis nitrogen regulatory PII protein.Protein science : a publication of the Protein Society · 2010Article
- Mutagenesis and functional characterization of the four domains of GlnD, a bifunctional nitrogen sensor protein.Journal of bacteriology · 2010Article
- Nitrogenase switch-off and regulation of ammonium assimilation in response to light deprivation in Rhodospirillum rubrum are influenced by the nitrogen source used during growth.Journal of bacteriology · 2010Article
- Mutations at pipX suppress lethality of PII-deficient mutants of Synechococcus elongatus PCC 7942.Journal of bacteriology · 2009Article
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Abstract
GlnD is a bifunctional uridylyltransferase/uridylyl-removing enzyme and is thought to be the primary sensor of nitrogen status in the cell. It plays an important role in nitrogen assimilation and metabolism by reversibly regulating the modification of P(II) proteins, which in turn regulate a variety of other proteins. We report here the characterization of glnD mutants from the photosynthetic, nitrogen-fixing bacterium Rhodospirillum rubrum and the analysis of the roles of GlnD in the regulation of nitrogen fixation. Unlike glnD mutations in Azotobacter vinelandii and some other bacteria, glnD deletion mutations are not lethal in R. rubrum. Such mutants grew well in minimal medium with glutamate as the sole nitrogen source, although they grew slowly with ammonium as the sole nitrogen source (MN medium) and were unable to fix N(2). The slow growth in MN medium is apparently due to low glutamine synthetase activity, because a DeltaglnD strain with an altered glutamine synthetase that cannot be adenylylated can grow well in MN medium. Various mutation and complementation studies were used to show that the critical uridylyltransferase activity of GlnD is localized to the N-terminal region. Mutants with intermediate levels of uridylyltransferase activity are differentially defective in nif gene expression, the posttranslational regulation of nitrogenase, and NtrB/NtrC function, indicating the complexity of the physiological role of GlnD. These results have implications for the interpretation of results obtained with GlnD in many other organisms.
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