ArticleBritish journal of cancer2005
Hypermethylation of the 5' CpG island of the gene encoding the serine protease Testisin promotes its loss in testicular tumorigenesis.
Article in British journal of cancer, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- A multi-analyte liquid biopsy approach for nonseminomatous testicular germ cell tumors: combining cfDNA and N-glycan analysis in blood and seminal plasma.Cancer cell international · 2025Article
- GPI-anchored serine proteases: essential roles in development, homeostasis, and disease.Biological chemistry · 2025Review
- Single-cell transcriptional profile of CD34+ hematopoietic progenitor cells from del(5q) myelodysplastic syndromes and impact of lenalidomide.Nature communications · 2024Article
- Promoter Hypomethylation of miR-124 Gene Is Associated With Major Depressive Disorder.Frontiers in molecular neuroscience · 2021Article
- The Relationship Between Microbiomes and Selective Regimes in the Sponge GenusFrontiers in microbiology · 2021Article
- Testisin/Prss21 deficiency causes increased vascular permeability and a hemorrhagic phenotype during luteal angiogenesis.PloS one · 2020Article
- Cell surface-anchored serine proteases in cancer progression and metastasis.Cancer metastasis reviews · 2019Review
- PRSS21/testisin inhibits ovarian tumor metastasis and antagonizes proangiogenic angiopoietins ANG2 and ANGPTL4.Journal of molecular medicine (Berlin, Germany) · 2019Article
- DNA Methylation and Flavonoids in Genitourinary Cancers.Current pharmacology reports · 2015Article
- Genome wide DNA methylation profiles provide clues to the origin and pathogenesis of germ cell tumors.PloS one · 2015Article
- Decreased expression of GRIM-19 by DNA hypermethylation promotes aerobic glycolysis and cell proliferation in head and neck squamous cell carcinoma.Oncotarget · 2015Article
- DNA methylation analysis reveals distinct methylation signatures in pediatric germ cell tumors.BMC cancer · 2013Article
- DNA methylation profiles delineate epigenetic heterogeneity in seminoma and non-seminoma.British journal of cancer · 2012Article
- Epigenetic: a molecular link between testicular cancer and environmental exposures.Frontiers in endocrinology · 2012Article
- Methylator phenotype of malignant germ cell tumours in children identifies strong candidates for chemotherapy resistance.British journal of cancer · 2011Article
- Membrane-anchored serine proteases in health and disease.Progress in molecular biology and translational science · 2011Review
- The cutting edge: membrane-anchored serine protease activities in the pericellular microenvironment.The Biochemical journal · 2010Review
- Discovery of DNA methylation markers in cervical cancer using relaxation ranking.BMC medical genomics · 2008Article
- Epigenetic silencing of the putative tumor suppressor gene testisin in testicular germ cell tumors.Journal of cancer research and clinical oncology · 2006Article
Corrections and comments
- Correction · 2015-12-01Concerns/Issues about Image · Manipulation of Images ·
- Erratum issued
Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Testisin gene (PRSS21) encodes a glycosylphosphatidylinositol (GPI)-linked serine protease that exhibits testis tissue-specific expression. Loss of Testisin has been implicated in testicular tumorigenesis, but its role in testis biology and tumorigenesis is not known. Here we have investigated the role of CpG methylation in Testisin gene inactivation and tested the hypothesis that Testisin may act as a tumour suppressor for testicular tumorigenesis. Using sequence analysis of bisulphite-treated genomic DNA, we find a strong relationship between hypermethylation of a 385 bp 5' CpG rich island of the Testisin gene, and silencing of the Testisin gene in a range of human tumour cell lines and in 100% (eight/eight) of testicular germ cell tumours. We show that treatment of Testisin-negative cell lines with demethylating agents and/or a histone deacetylase inhibitor results in reactivation of Testisin gene expression, implicating hypermethylation in Testisin gene silencing. Stable expression of Testisin in the Testisin-negative Tera-2 testicular cancer line suppressed tumorigenicity as revealed by inhibition of both anchorage-dependent cell growth and tumour formation in an SCID mouse model of testicular tumorigenesis. Together, these data show that loss of Testisin is caused, at least in part, by DNA hypermethylation and histone deacetylation, and suggest a tumour suppressor role for Testisin in testicular tumorigenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.