ArticleThe Journal of experimental medicine2005
Anti-CD63 antibodies suppress IgE-dependent allergic reactions in vitro and in vivo.
Article in The Journal of experimental medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Alpha-gal-specific human IgE promotes both alpha-gal-induced and antigen-independent allergic effector cell activation.The Journal of allergy and clinical immunology · 2026Article
- Stabilized COre gene and Pathway Election uncovers pan-cancer shared pathways and a cancer-specific driver.Science advances · 2022Article
- Distinct Small Intestine Mast Cell Histologic Changes in Patients With Hereditary Alpha-tryptasemia and Mast Cell Activation Syndrome.The American journal of surgical pathology · 2021Article
- Tetraspanins in the regulation of mast cell function.Medical microbiology and immunology · 2020Review
- Article
- Phosphoproteomic Landscaping Identifies Non-canonical cKIT Signaling in Polycythemia Vera Erythroid Progenitors.Frontiers in oncology · 2019Article
- CD9 Tetraspanin: A New Pathway for the Regulation of Inflammation?Frontiers in immunology · 2018Review
- Video-Rate Bioluminescence Imaging of Degranulation of Mast Cells Attached to the Extracellular Matrix.Frontiers in cell and developmental biology · 2018Article
- Tetraspanins and Transmembrane Adaptor Proteins As Plasma Membrane Organizers-Mast Cell Case.Frontiers in cell and developmental biology · 2016Review
- Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases.The World Allergy Organization journal · 2016Review
- Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Mast cells and basophils are essential for allergies: mechanisms of allergic inflammation and a proposed procedure for diagnosis.Acta pharmacologica Sinica · 2013Review
- The tetraspanin CD63 is required for efficient IgE-mediated mast cell degranulation and anaphylaxis.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Tetraspanins in mast cells.Frontiers in immunology · 2012Article
- Expression of CD203c and CD63 in human basophils: relationship to differential regulation of piecemeal and anaphylactic degranulation processes.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2010Article
- Adapters in the organization of mast cell signaling.Immunological reviews · 2009Review
- The first transmembrane region of the beta-chain stabilizes the tetrameric Fc epsilon RI complex.Molecular immunology · 2009Article
- Deficiency of the tetraspanin CD63 associated with kidney pathology but normal lysosomal function.Molecular and cellular biology · 2009Article
- Treatment strategies for allergy and asthma.Nature reviews. Immunology · 2008Review
- The long and arduous road to CRAC.Cell calcium · 2007Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
High-affinity IgE receptor (FcepsilonRI) cross-linking on mast cells (MCs) induces secretion of preformed allergy mediators (degranulation) and synthesis of lipid mediators and cytokines. Degranulation produces many symptoms of immediate-type allergic reactions and is modulated by adhesion to surfaces coated with specific extracellular matrix (ECM) proteins. The signals involved in this modulation are mostly unknown and their contribution to allergic reactions in vivo is unclear. Here we report the generation of monoclonal antibodies that potently suppress FcepsilonRI-induced degranulation, but not leukotriene synthesis. We identified the antibody target as the tetraspanin CD63. Tetraspanins are membrane molecules that form multimolecular complexes with a broad array of molecules including ECM protein-binding beta integrins. We found that anti-CD63 inhibits MC adhesion to fibronectin and vitronectin. Furthermore, anti-CD63 inhibits FcepsilonRI-mediated degranulation in cells adherent to those ECM proteins but not in nonadherent cells. Thus the inhibition of degranulation by anti-CD63 correlates with its effect on adhesion. In support of a mechanistic linkage between the two types of inhibition, anti-CD63 had no effect on FcepsilonRI-induced global tyrosine phosphorylation and calcium mobilization but impaired the Gab2-PI3K pathway that is known to be essential for both degranulation and adhesion. Finally, we showed that these antibodies inhibited FcepsilonRI-mediated allergic reactions in vivo. These properties raise the possibility that anti-CD63 could be used as therapeutic agents in MC-dependent diseases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.