ArticleBiophysical journal2005
A model-independent algorithm to derive Ca2+ fluxes underlying local cytosolic Ca2+ transients.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Biological physics to uncover cell signaling.Biophysical reviews · 2025Review
- High rates of calcium-free diffusion in the cytosol of living cells.Biophysical journal · 2021Article
- Article
- Inverse problems in neuronal calcium signaling.Journal of mathematical biology · 2013Article
- Mean field strategies induce unrealistic non-linearities in calcium puffs.Frontiers in physiology · 2011Article
- Recording single-channel activity of inositol trisphosphate receptors in intact cells with a microscope, not a patch clamp.The Journal of general physiology · 2010Article
- Article
- Multi-dimensional resolution of elementary Ca2+ signals by simultaneous multi-focal imaging.Cell calcium · 2008Article
- The number and spatial distribution of IP3 receptors underlying calcium puffs in Xenopus oocytes.Biophysical journal · 2006Article
- Analysis of puff dynamics in oocytes: interdependence of puff amplitude and interpuff interval.Biophysical journal · 2006Article
- "Optical patch-clamping": single-channel recording by imaging Ca2+ flux through individual muscle acetylcholine receptor channels.The Journal of general physiology · 2005Article
- Imaging single-channel calcium microdomains.Cell calciumReview
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Local intracellular Ca(2+) signals result from Ca(2+) flux into the cytosol through individual channels or clusters of channels. To gain a mechanistic understanding of these events we need to know the magnitude and spatial distribution of the underlying Ca(2+) flux. However, this is difficult to infer from fluorescence Ca(2+) images because the distribution of Ca(2+)-bound dye is affected by poorly characterized processes including diffusion of Ca(2+) ions, their binding to mobile and immobile buffers, and sequestration by Ca(2+) pumps. Several methods have previously been proposed to derive Ca(2+) flux from fluorescence images, but all require explicit knowledge or assumptions regarding these processes. We now present a novel algorithm that requires few assumptions and is largely model-independent. By testing the algorithm with both numerically generated image data and experimental images of sparklets resulting from Ca(2+) flux through individual voltage-gated channels, we show that it satisfactorily reconstructs the magnitude and time course of the underlying Ca(2+) currents.
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Registered trials
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