ArticleJournal of virology2005
RNA recombination of hepatitis delta virus in natural mixed-genotype infection and transfected cultured cells.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Sequence analysis of the hepatitis D virus across genotypes reveals highly conserved regions amidst evidence of recombination.Virus evolution · 2025Article
- Article
- Strong Replication Interference Between Hepatitis Delta Viruses in Human Liver Chimeric Mice.Frontiers in microbiology · 2021Article
- The evolution and clinical impact of hepatitis B virus genome diversity.Nature reviews. Gastroenterology & hepatology · 2020Review
- Structural Pattern Differences in Unbranched Rod-like RNA of Hepatitis Delta Virus affect RNA Editing.Viruses · 2019Article
- Recombinant identification, molecular classification and proposed reference genomes for hepatitis delta virus.Journal of viral hepatitis · 2019Article
- Article
- Hepatitis delta: virological and clinical aspects.Virology journal · 2017Review
- Is Hepatitis Delta infections important in Brazil?BMC infectious diseases · 2016Article
- Cell type-dependent RNA recombination frequency in the Japanese encephalitis virus.BioMed research international · 2014Article
- Evolution and diversity of the human hepatitis d virus genome.Advances in bioinformatics · 2010Article
- Insights into the single-cell reproduction cycle of members of the family Bromoviridae: lessons from the use of protoplast systems.Journal of virology · 2008Review
- Quasispecies and its impact on viral hepatitis.Virus research · 2007Review
- Evolution of hepatitis delta virus RNA genome following long-term replication in cell culture.Journal of virology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most RNA viruses encode their own RNA polymerases for genome replication, and increasing numbers of them appear to be capable of undergoing RNA recombination. Here, we provide the first report of intergenotypic recombination in hepatitis delta virus (HDV), the only animal RNA virus that requires host RNA polymerase(s) for viral replication. In vivo, we analyzed RNA species derived from the serum of a patient with mixed genotype I and genotype IIb HDV infection by using multiple restriction fragment length polymorphism (RFLP) assays and sequence analysis of cloned reverse transcription (RT)-PCR products. Six HDV recombinants were isolated from 101 tested clones, and HDV recombination in this patient was further confirmed by RT-PCR with genotype-specific primer pairs. Analysis of the recombination junctions suggested that the HDV genome rearrangement occurred through faithful homologous recombination. We then used an RNA cotransfection cell culture system to investigate HDV RNA recombination in vitro. We found that HDV recombinants could indeed be detected in the transfected cells; some of these possessed recombination junctions identical to those identified in vivo. Furthermore, using a PCR-independent RNase protection assay, we were able to readily identify the recombined HDV RNA species in cultured cells. Taken together, our results demonstrate that HDV RNA recombination occurs in both natural HDV infections and cultured cells, thereby presenting a novel mechanism for HDV evolution.
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