ArticleEMBO reports2005
Redefining the subcellular location and transport of APC: new insights using a panel of antibodies.
Article in EMBO reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 58 citations in OpenAlex.
- Manipulation of Wnt Signaling Pathway during Peripheral Nerve Regeneration.Cellular and molecular neurobiology · 2026Article
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- Inhibition of Drp1 SUMOylation by ALR protects the liver from ischemia-reperfusion injury.Cell death and differentiation · 2021Article
- Oncogenic Serine 45-Deleted β-Catenin Remains Susceptible to Wnt Stimulation and APC Regulation in Human Colonocytes.Cancers · 2020Article
- Validation and application of a novel APC antibody in western blotting, immunoprecipitation, and immunohistochemistry.Medical molecular morphology · 2018Article
- Insulin signaling regulates a functional interaction between adenomatous polyposis coli and cytoplasmic dynein.Molecular biology of the cell · 2017Article
- Wnt/β-catenin signaling plays an ever-expanding role in stem cell self-renewal, tumorigenesis and cancer chemoresistance.Genes & diseases · 2016Article
- Immunopurification of adenomatous polyposis coli (APC) proteins.BMC research notes · 2013Article
- The β-catenin destruction complex.Cold Spring Harbor perspectives in biology · 2013Review
- Identifying novel protein complexes in cancer cells using epitope-tagging of endogenous human genes and affinity-purification mass spectrometry.Journal of proteome research · 2012Article
- Actin nucleators in the nucleus: an emerging theme.Journal of cell science · 2012Review
- Cortical localization of APC2 plays a role in actin organization but not in Wnt signaling in Drosophila.Journal of cell science · 2011Article
- EB1 is required for spindle symmetry in mammalian mitosis.PloS one · 2011Article
- Adenomatous polyposis coli regulates axon arborization and cytoskeleton organization via its N-terminus.PloS one · 2011Article
- TCF-3, 4 protein expression correlates with beta-catenin expression in MSS and MSI-H colorectal cancer from HNPCC patients but not in sporadic colorectal cancers.International journal of colorectal disease · 2010Article
- Novel association of APC with intermediate filaments identified using a new versatile APC antibody.BMC cell biology · 2009Article
- Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase.The Journal of biological chemistry · 2009Article
- Nuclear APC.Advances in experimental medicine and biology · 2009Review
- Truncation mutations abolish chromatin-associated activities of adenomatous polyposis coli.Oncogene · 2008Article
- The APC tumor suppressor inhibits DNA replication by directly binding to DNA via its carboxyl terminus.Gastroenterology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adenomatous polyposis coli (APC) is a tumour suppressor involved in colon cancer progression. We and others previously described nuclear-cytoplasmic shuttling of APC. However, there are conflicting reports concerning the localization of endogenous wild-type and tumour-associated, truncated APC. To resolve this issue, we compared APC localization using immunofluorescence (IF) microscopy and cell fractionation with nine different APC antibodies. We found that three commonly used APC antibodies showed nonspecific nuclear staining by IF and validated this conclusion in cells where APC was inactivated using small interfering RNA or Cre/Flox. Fractionation showed that wild-type and truncated APC from colon cancer cells were primarily cytoplasmic, but increased in the nucleus after leptomycin B treatment, consistent with CRM1-dependent nuclear export. In contrast to recent reports, our biochemical data indicate that APC nuclear localization is not regulated by changes in cell density, and that APC nuclear export is not prevented by truncating mutations in cancer. These results verify that the bulk of APC resides in the cytoplasm and indicate the need for caution when evaluating the nuclear accumulation of APC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.