ArticleProceedings of the National Academy of Sciences of the United States of America2005
Three GnRH receptor types in laser-captured single cells of the cichlid pituitary display cellular and functional heterogeneity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Differential Regulation of Gonadotropins as Revealed by Transcriptomes of Distinct LH and FSH Cells of Fish Pituitary.International journal of molecular sciences · 2021Article
- Article
- Morphological Evidence for Functional Crosstalk Between Multiple GnRH Systems in the Male Tilapia,Frontiers in endocrinology · 2020Article
- A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel.Frontiers in endocrinology · 2018Article
- Functional significance of GnRH and kisspeptin, and their cognate receptors in teleost reproduction.Frontiers in endocrinology · 2013Article
- Fish and chips: functional genomics of social plasticity in an African cichlid fish.The Journal of experimental biology · 2008Article
- GnRH and gpcr: laser-captured single cell gene profiling.Fish physiology and biochemistry · 2005Article
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3 authors.
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Abstract
The role of multiple gonadotropin-releasing hormone receptor (GnRH-R) types in the regulation of gonadotropic and nongonadotropic cells remains speculative. To address this issue, we developed a technology integrating laser-captured microdissection of single digoxigenin-labeled pituitary cells coupled with real-time quantitative PCR to examine the expression profiles of three endogenous GnRH-R types (R1, R2, and R3) in immature and mature males of tilapia Oreochromis niloticus. Here, in addition to gonadotropes (luteinizing and folicle-stimulating hormone, FSH), we show GnRH-Rs are also present in lactotropes, somatotropes, thyrotropes, melanotropes (melanocyte-stimulating hormone, MSH), corticotropes and somatolactin cells. Subpopulations of pituitary cells express single (42.9%), multiple (32.4%) or lack (24.7%) GnRH-Rs. In immature males, the percentage of FSH cells containing combinations of GnRH-Rs was significantly higher (R1+R2: 24%, P <0.05; R1+R2+R3: 25%, P <0.01) than in mature males, whereas the percentage showing only R1 and R1 and R3 transcripts (P <0.05) was higher in mature males. Significantly greater copies of R1 and R3 transcripts were found in MSH cells of immature and mature males, respectively (P <0.05). GnRH-R transcripts in other pituitary cells (lactotropes, R1 and R2; somatolactin cells/thyrotropes/corticotropes, R1, R2, and R3) were significantly higher in mature males (P <0.05) but were unaltered in somatotropes and luteinizing hormone cells. Thus, FSH and MSH cells are required for both reproductive states, whereas other pituitary cells are recruited only during testicular maturation. The differential expression of GnRH-Rs in gonadotropic and nongonadotropic cells demonstrates cellular and functional heterogeneity of mechanisms controlling normal sexual development.
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