ArticleProceedings of the National Academy of Sciences of the United States of America2005
Targeted mutagenesis using zinc-finger nucleases in Arabidopsis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 143 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
143 citing papers in PubMed, 415 citations in OpenAlex.
- Research progress in gene editing of Brassicaceae crops.Biodesign research · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- AI-assisted crop improvement: new design tools within established regulatory frameworks.Frontiers in plant science · 2026Article
- From convention to innovation: the role of genetic modification and genome editing in Australian wheat breeding.AoB PLANTS · 2025Review
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Cotton under heat stress: a comprehensive review of molecular breeding, genomics, and multi-omics strategies.Frontiers in genetics · 2025Review
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Genome editing in Sub-Saharan Africa: a game-changing strategy for climate change mitigation and sustainable agriculture.GM crops & food · 2024Review
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production.Frontiers in genome editing · 2024Review
- Principles and practice of virus induced gene silencing for functional genomics in plants.Virus genes · 2023Review
- Review
- The Role of Italy in the Use of Advanced Plant Genomic Techniques on Fruit Trees: State of the Art and Future Perspectives.International journal of molecular sciences · 2023Review
- The genusFrontiers in plant science · 2023Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- CRISPR-Based Genome Editing and Its Applications in Woody Plants.International journal of molecular sciences · 2022Review
- Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs inJournal of fungi (Basel, Switzerland) · 2022Article
- CRISPR/Cas9 Technique for Temperature, Drought, and Salinity Stress Responses.Current issues in molecular biology · 2022Review
- Expanding Gene-Editing Potential in Crop Improvement with Pangenomes.International journal of molecular sciences · 2022Review
- Comprehending the evolution of gene editing platforms for crop trait improvement.Frontiers in genetics · 2022Review
83 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Targeted mutagenesis is an essential tool of reverse genetics that could be used experimentally to investigate basic plant biology or modify crop plants for improvement of important agricultural traits. Although targeted mutagenesis is routine in several model organisms including yeast and mouse, efficient and widely usable methods to generate targeted modifications in plant genes are not currently available. In this study we investigated the efficacy of a targeted-mutagenesis approach based on zinc-finger nucleases (ZFNs). In this procedure, ZFNs are used to generate double-strand breaks at specific genomic sites, and subsequent repair produces mutations at the break site. To determine whether ZFNs can cleave and induce mutations at specific sites within higher plant genomes, we introduced a construct carrying both a ZFN gene, driven by a heat-shock promoter, and its target into the Arabidopsis genome. Induction of ZFN expression by heat shock during seedling development resulted in mutations at the ZFN recognition sequence at frequencies as high as 0.2 mutations per target. Of 106 ZFN-induced mutations characterized, 83 (78%) were simple deletions of 1-52 bp (median of 4 bp), 14 (13%) were simple insertions of 1-4 bp, and 9 (8%) were deletions accompanied by insertions. In 10% of induced individuals, mutants were present in the subsequent generation, thus demonstrating efficient transmission of the ZFN-induced mutations. These data indicate that ZFNs can form the basis of a highly efficient method for targeted mutagenesis of plant genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.