ArticleMammalian genome : official journal of the International Mammalian Genome Society2005
Assessment of a systematic expression profiling approach in ENU-induced mouse mutant lines.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed, 23 citations in OpenAlex.
- Phenotyping structural abnormalities in mouse embryos using high-resolution episcopic microscopy.Disease models & mechanisms · 2014Article
- Pleiotropic effects in Eya3 knockout mice.BMC developmental biology · 2008Article
- Episcopic 3D Imaging Methods: Tools for Researching Gene Function.Current genomics · 2008Article
- Transcriptional profiling of murine organ genes in response to infection with Bacillus anthracis Ames spores.Microbial pathogenesis · 2008Article
- Expression profiling of the mouse early embryo: reflections and perspectives.Developmental dynamics : an official publication of the American Association of Anatomists · 2006Review
- Identification of coexpressed gene clusters in a comparative analysis of transcriptome and proteome in mouse tissues.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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Abstract
Comparative genomewide expression profiling is a powerful tool in the effort to annotate the mouse genome with biological function. The systematic analysis of RNA expression data of mouse lines from the Munich ENU mutagenesis screen might support the understanding of the molecular biology of such mutants and provide new insights into mammalian gene function. In a direct comparison of DNA microarray experiments of individual versus pooled RNA samples of organs from ENU-induced mouse mutants, we provide evidence that individual RNA samples may outperform pools in some aspects. Genes with high biological variability in their expression levels (noisy genes) are identified as false positives in pooled samples. Evidence suggests that highly stringent housing conditions and standardized procedures for the isolation of organs significantly reduce biological variability in gene expression profiling experiments. Data on wild-type individuals demonstrate the positive effect of controlling variables such as social status, food intake before organ sampling, and stress with regard to reproducibility of gene expression patterns. Analyses of several organs from various ENU-induced mutant lines in general show low numbers of differentially expressed genes. We demonstrate the feasibility to detect transcriptionally affected organs employing RNA expression profiling as a tool for molecular phenotyping.
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