ArticleInflammation2004
Native versus recombinant high-mobility group B1 proteins: functional activity in vitro.
Article in Inflammation, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- CpG-A induces liquid-liquid phase separation of HMGB1 to activate the RAGE-mediated inflammatory pathway.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Antibody and Protein Profiles in Glaucoma: Screening of Biomarkers and Identification of Signaling Pathways.Biology · 2021Review
- The performance of the alarmin HMGB1 in pediatric diseases: From lab to clinic.Immunity, inflammation and disease · 2021Review
- HMGB1: meeting the need for new tools in the box.Mucosal immunology · 2019Article
- Hepatocellular carcinoma-associated antigen 59 of Haemonchus contortus modulates the functions of PBMCs and the differentiation and maturation of monocyte-derived dendritic cells of goats in vitro.Parasites & vectors · 2019Article
- High mobility group box-1 induces pro-inflammatory signaling in human nucleus pulposus cells via toll-like receptor 4-dependent pathway.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Secreted HMGB1 from Wnt activated intestinal cells is required to maintain a crypt progenitor phenotype.Oncotarget · 2016Article
- HMGB1 in health and disease.Molecular aspects of medicine · 2014Review
- Screening for antifibrotic compounds using high throughput system based on fluorescence polarization.Biology · 2014Article
- The translocation of nuclear molecules during inflammation and cell death.Antioxidants & redox signaling · 2014Review
- Armed response: how dying cells influence T-cell functions.Immunological reviews · 2011Review
- Preconditioning with high mobility group box 1 (HMGB1) induces lipoteichoic acid (LTA) tolerance.Journal of immunotherapy (Hagerstown, Md. : 1997) · 2010Article
- HMGb1 promotes scratch wound closure of HaCaT keratinocytes via ERK1/2 activation.Molecular and cellular biochemistry · 2009Article
- Alarmins link neutrophils and dendritic cells.Trends in immunology · 2009Review
- Preconditioning with high mobility group box 1 (HMGB1) induces lipopolysaccharide (LPS) tolerance.Journal of leukocyte biology · 2008Article
- A high-sensitivity method for detection and measurement of HMGB1 protein concentration by high-affinity binding to DNA hemicatenanes.PloS one · 2008Article
- The inflammatory response to cell death.Annual review of pathology · 2008Review
- High-mobility group box protein 1 (HMGB1): an alarmin mediating the pathogenesis of rheumatic disease.Arthritis research & therapy · 2008Review
- The role of cell death in the pathogenesis of autoimmune disease: HMGB1 and microparticles as intercellular mediators of inflammation.Modern rheumatology · 2008Review
- Bench-to-bedside review: High-mobility group box 1 and critical illness.Critical care (London, England) · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To compare the functional activity of native HMGB1 proteins from eukaryotic sources with HMGB1 from prokaryotic sources the cDNAs of human and murine HMGB1 were cloned and the proteins expressed in bacteria. Tissue-derived HMGB1 from calf thymus and HMGB1 secreted from Chinese hamster ovary (CHO) cells were purified. Human whole blood, THP-1 cells, and NIH/3T3 cells were exposed to HMGB1 proteins and the induction of tumor necrosis factor-alpha (TNF-alpha) release in whole blood and monocytic THP-1 cells and a proliferation assay in NIH/3T3 cells were used to study functional activity of HMGB1s in vitro. Native and recombinant HMGB1s induced TNF-alpha release in human blood and in THP-1 cells dose-dependently, but recombinant HMGB1s were more effective. Cell proliferation was induced by native and recombinant HMGB1s. The native HMGB1 proteins from eukaryotic sources exert the same (though less pronounced) biological activity in vitro as recombinant HMGB1 proteins from prokaryotic sources.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.