ArticleClinical & experimental metastasis2004
Experimental liver metastasis: standards for local cell implantation to study isolated tumor growth in mice.
Article in Clinical & experimental metastasis, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
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- Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model.International journal of molecular sciences · 2024Article
- PD-1/CTLA-4 Blockade Leads to Expansion of CD8Liver cancer · 2023Article
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- Bioactive phospho-therapy with black phosphorus forTheranostics · 2020Article
- Impact of surgical extent and sex on the hepatic metastasis of colon cancer.Surgery today · 2014Article
- The phosphodiesterase 3 inhibitor cilostazol does not stimulate growth of colorectal liver metastases after major hepatectomy.Clinical & experimental metastasis · 2014Article
- Changes of T cells and cytokines TGF-β1 and IL-10 in mice during liver metastasis of colon carcinoma: implications for liver anti-tumor immunity.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2013Article
- A novel animal model for in vivo study of liver cancer metastasis.World journal of gastroenterology · 2012Article
- Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases through the Action of Stromal Cell-Derived Factor-1.Journal of oncology · 2012Article
- Preferential accumulation within tumors and in vivo imaging by functionalized luminescent dendrimer lanthanide complexes.Biomaterials · 2011Article
- Luminescence targeting and imaging using a nanoscale generation 3 dendrimer in an in vivo colorectal metastatic rat model.Nanomedicine : nanotechnology, biology, and medicine · 2011Article
- Vascular profile characterization of liver tumors by magnetic resonance imaging using hemodynamic response imaging in mice.Neoplasia (New York, N.Y.) · 2011Article
- Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.BMC cancer · 2010Article
- The intraportal injection model: a practical animal model for hepatic metastases and tumor cell dissemination in human colon cancer.BMC cancer · 2009Article
- Stromal cell-derived factor-1 promotes cell migration and tumor growth of colorectal metastasis.Neoplasia (New York, N.Y.) · 2007Article
- Modeling the behavior of uveal melanoma in the liver.Investigative ophthalmology & visual science · 2007Article
- Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model.BMC cancer · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Experimental hepatic metastasis of colorectal tumors is frequently studied by local intrahepatic tumor cell implantation. However, although a variety of factors of the implantation procedure may markedly influence tumor growth characteristics, standards are not defined yet. Herein, we studied the effect of different modes of cell implantation on tumor growth and angiogenesis by in vivo fluorescence microscopy and histology seven days after grafting colorectal CT26.WT tumor cells into the left liver lobe of syngeneic BALB/c mice. We demonstrate that (i) radial growth of cells implanted within the central area of the lobe is inhibited by a regularly observed fissura which crosses at midline the surface of the lobe; (ii) cells suspended during implantation in RPMI show an uncontrolled overwhelming growth 40-fold of those suspended in PBS; (iii) cell implantation in 100 microl and 20 microl suspension medium is significantly more complicated by rupture of the liver capsule, uncontrolled intraparenchymal cell spread and recoil of the cells through the injection canal compared to cells suspended in 10 microl; (iv) the frequency of metastasis within the injection canal and at the puncture site is significantly reduced using 32G compared to 27G or 29G needles; (v) occlusion of the puncture site by acrylic glue or electric coagulation completely abolishes peritoneal tumor spread compared to no treatment or gentle compression by cotton gauze. We conclude that a standardized growth of isolated metastases is best achieved by implanting CT26.WT cells in a 10 microl PBS blister subcapsularly into the paramedian area of the lower surface of the left liver lobe, using a 32-gauge needle and closing the puncture site with acrylic glue.
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