ArticleTransgenic research2004
Stable transformation of petunia plastids.
Article in Transgenic research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Development of chloroplast transformation for five species in the genus Nicotiana.The Plant journal : for cell and molecular biology · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Heterologous expression of Arabidopsis SOS3 increases salinity tolerance in Petunia.Molecular biology reports · 2022Article
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Paternal inheritance of plastid-encoded transgenes inBiotechnology reports (Amsterdam, Netherlands) · 2017Article
- Expression of a Codon-Optimized dsdA Gene in Tobacco Plastids and Rice Nucleus Confers D-Serine Tolerance.Frontiers in plant science · 2016Article
- Low frequency paternal transmission of plastid genes in Brassicaceae.Transgenic research · 2015Article
- Article
- Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine.Plant physiology · 2012Article
- Plastid genetic engineering in Solanaceae.Protoplasma · 2012Review
- Plastid biotechnology for crop production: present status and future perspectives.Plant molecular biology · 2011Article
- Plastid biotechnology: food, fuel, and medicine for the 21st century.Plant physiology · 2011Review
- High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.Transgenic research · 2011Article
- Plant plastid engineering.Current genomics · 2010Article
- Plastid transformation in eggplant (Solanum melongena L.).Transgenic research · 2010Article
- Comparative analysis of the complete sequence of the plastid genome of Parthenium argentatum and identification of DNA barcodes to differentiate Parthenium species and lines.BMC plant biology · 2009Article
- Introduction of transformed chloroplasts from tobacco into petunia by asymmetric cell fusion.Plant cell reports · 2009Article
- High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts.Transgenic research · 2009Article
- Transfer of plastid DNA to the nucleus is elevated during male gametogenesis in tobacco.Plant physiology · 2008Article
- Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins.Transgenic research · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastid transformation results in stably expressed foreign genes, which for most Angiosperms are largely excluded from sperm cells, thereby greatly reducing the risk of foreign gene spread through pollen. Prior to this work, fertile plastid transformants were restricted to tobacco, tomato and Lesquerella. Application of plastid engineering in the important floriculture industry requires the development of stable plastid transformation in a major ornamental plant species such as Petunia hybrida. Here we describe the successful isolation of fertile and stable plastid transformants in a commercial cultivar of P. hybrida (var. Pink Wave). Plastid targeting regions from tobacco were used to integrate aadA and gusA between the accD and rbcL genes of P. hybrida plastid DNA following particle bombardment of leaves. For three spectinomycin and streptomycin resistant lines, DNA blot analysis confirmed transgene integration into plastid DNA and homoplasmy. Maternal inheritance and homoplasmy resulted in 100% transmission of spectinomycin resistance to progeny after selfing. Plastid transformants expressed the gusA gene uniformly within leaves and to comparable levels in all three lines. Insertion of trait genes in place of gusA coding sequences enables immediate applications of our plastid transformation vector. Establishment of plastid transformation in P. hybrida facilitates a safe and reliable use of this important ornamental plant for research and plant biotechnology.
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Registered trials
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