Evidence map›Paper›PMID 15670753›Full record

ArticleBiochemical and biophysical research communications2005

Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films.

Joanna Lukomska, Ignacy Gryczynski, Joanna Malicka, Slawomir Makowiec, Joseph R Lakowicz, Zygmunt Gryczynski

Abstract readComparative StudyEvaluation StudyValidation Study
In one paragraph

Article in Biochemical and biophysical research communications, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Joanna LukomskaCenter for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA. joanna@cfs.umbi.umd.edu
Ignacy Gryczynski
Joanna Malicka
Slawomir Makowiec
Joseph R Lakowicz
Zygmunt Gryczynski

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119
6 · The paper itself

Abstract

We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.

Indexed as

CarbocyaninesBuffersCoated Materials, BiocompatibleDNAMicroscopy, Fluorescence, MultiphotonReproducibility of ResultsSensitivity and SpecificitySilverStaining and LabelingBuffersCarbocyaninesCoated Materials, Biocompatiblecyanine dye 3cyanine dye 5DNASilver

Identifiers

PMID15670753
PMCPMC6844251

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.