ArticleFunctional & integrative genomics2005
Direct targeting and rapid isolation of BAC clones spanning a defined chromosome region.
Article in Functional & integrative genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch.Nature communications · 2021Article
- Gene Expression Profiling and Fine Mapping Identifies a Gibberellin 2-Oxidase Gene Co-segregating With the Dominant Dwarfing GeneFrontiers in plant science · 2019Article
- Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition.Journal of experimental botany · 2017Article
- A Metabolic Gene Cluster in the Wheat W1 and the Barley Cer-cqu Loci Determines β-Diketone Biosynthesis and Glaucousness.The Plant cell · 2016Article
- Haplotype divergence and multiple candidate genes at Rphq2, a partial resistance QTL of barley to Puccinia hordei.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- Comparative functional analysis of two wheat Na(+)/H (+) antiporter SOS1 promoters in Arabidopsis thaliana under various stress conditions.Journal of applied genetics · 2015Article
- Major haplotype divergence including multiple germin-like protein genes, at the wheat Sr2 adult plant stem rust resistance locus.BMC plant biology · 2014Article
- A high density physical map of chromosome 1BL supports evolutionary studies, map-based cloning and sequencing in wheat.Genome biology · 2013Article
- A dominant point mutation in a RINGv E3 ubiquitin ligase homoeologous gene leads to cleistogamy in Brassica napus.The Plant cell · 2012Article
- New cis-regulatory elements in the Rht-D1b locus region of wheat.Functional & integrative genomics · 2012Article
- BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.).BMC genomics · 2011Article
- Combining genetical genomics and bulked segregant analysis-based differential expression: an approach to gene localization.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Fine mapping, physical mapping and development of diagnostic markers for the Rrs2 scald resistance gene in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Article
- Physical analysis of the complex rye (Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Analysis of intraspecies diversity in wheat and barley genomes identifies breakpoints of ancient haplotypes and provides insight into the structure of diploid and hexaploid triticeae gene pools.Plant physiology · 2009Article
- Barley genomics: An overview.International journal of plant genomics · 2008Article
- A 1,000-loci transcript map of the barley genome: new anchoring points for integrative grass genomics.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007Article
- Generation and screening of a BAC library from a diploid potato clone to unravel durable late blight resistance on linkage group IV.International journal of plant genomics · 2007Article
- Chromosome-based genomics in the cereals.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To isolate genes of interest in plants, it is essential to construct bacterial artificial chromosome (BAC) libraries from specific genotypes. Construction and organisation of BAC libraries is laborious and costly, especially from organisms with large and complex genomes. In the present study, we developed the pooled BAC library strategy that allows rapid and low cost generation and screening of genomic libraries from any genotype of interest. The BAC library is constructed, directly organised into a few pools and screened for BAC clones of interest using PCR and hybridisation steps, without requiring organization into individual clones. As a proof of concept, a pooled BAC library of approximately 177,000 recombinant clones has been constructed from the barley cultivar Cebada Capa that carries the Rph7 leaf rust resistance gene. The library has an average insert size of 140 kb, a coverage of six barley genome equivalents and is organised in 138 pools of about 1,300 clones each. We rapidly established a single contig of six BAC clones spanning 230 kb at the Rph7 locus on chromosome 3HS. The described low-cost cloning strategy is fast and will greatly facilitate direct targeting of genes and large-scale intra- and inter-species comparative genome analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.