ArticlePlant physiology2005
Tonoplast intrinsic proteins AtTIP2;1 and AtTIP2;3 facilitate NH3 transport into the vacuole.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers, 1 of them a synthesis that pooled it.
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Who cites it
115 citing papers in PubMed, 1 synthesis or guideline pooled it, 335 citations in OpenAlex.
- Plant ammonium sensitivity is associated with external pH adaptation, repertoire of nitrogen transporters, and nitrogen requirement.Journal of experimental botany · 2024Pooled it
- Comprehensive DIA-MS Proteomics of Root Basal Nodes Elucidates Mechanisms of Salt Tolerance in Rice.Proteomics · 2026Article
- A Semi-Quantitative Yeast Complementation Platform for Characterizing Urea and Ammonia Transport by Membrane Channels.Current protocols · 2026Article
- Homeostasis of ammonium and nitrate in plants.Quantitative plant biology · 2026Review
- The evolution of the aquaporin gene family and drought tolerance mechanisms in green plants.Horticulture research · 2025Article
- Root remodeling mechanisms and salt tolerance trade-offs: The roles of HKT1, TMAC2, and TIP2;2 in Arabidopsis.PLoS genetics · 2025Article
- Yeast Complementation Assays Demonstrating the Importance of the Affinity Tag Position in Membrane Protein Purification, as Exemplified bySmall science · 2025Article
- OsPIP2;4 aquaporin water channel primarily expressed in roots of rice mediates both water and nonselective NaScientific reports · 2025Article
- Ammonium Transporter 1 (Current issues in molecular biology · 2025Article
- Advances in the Structure, Function, and Regulatory Mechanism of Plant Plasma Membrane Intrinsic Proteins.Genes · 2024Review
- TIP aquaporins in Cyperus esculentus: genome-wide identification, expression profiles, subcellular localizations, and interaction patterns.BMC plant biology · 2024Article
- AtPIP1;4 and AtPIP2;4 Cooperatively Mediate HPlants (Basel, Switzerland) · 2024Article
- Article
- Analysis ofPlants (Basel, Switzerland) · 2023Article
- Impaired cell growth under ammonium stress explained by modeling the energy cost of vacuole expansion in tomato leaves.The Plant journal : for cell and molecular biology · 2022Article
- Ubiquitin ligase OsRINGzf1 regulates drought resistance by controlling the turnover of OsPIP2;1.Plant biotechnology journal · 2022Article
- Label-free quantitative proteomics of maize roots from different root zones provides insight into proteins associated with enhance water uptake.BMC genomics · 2022Article
- Genome-wide identification and expression analysis of ammonium transporter 1 (AMT1) gene family in cassava (3 Biotech · 2022Article
- Zinc Transporter ZmLAZ1-4 Modulates Zinc Homeostasis on Plasma and Vacuolar Membrane in Maize.Frontiers in plant science · 2022Article
- Genome-Wide Identification, Structure Characterization, and Expression Pattern Profiling of the Aquaporin Gene Family inInternational journal of molecular sciences · 2021Article
55 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While membrane transporters mediating ammonium uptake across the plasma membrane have been well described at the molecular level, little is known about compartmentation and cellular export of ammonium. (The term ammonium is used to denote both NH3 and NH4+ and chemical symbols are used when specificity is required.) We therefore developed a yeast (Saccharomyces cerevisiae) complementation approach and isolated two Arabidopsis (Arabidopsis thaliana) genes that conferred tolerance to the toxic ammonium analog methylammonium in yeast. Both genes, AtTIP2;1 and AtTIP2;3, encode aquaporins of the tonoplast intrinsic protein subfamily and transported methylammonium or ammonium in yeast preferentially at high medium pH. AtTIP2;1 expression in Xenopus oocytes increased 14C-methylammonium accumulation with increasing pH. AtTIP2;1- and AtTIP2;3-mediated methylammonium detoxification in yeast depended on a functional vacuole, which was in agreement with the subcellular localization of green fluorescent protein-fusion proteins on the tonoplast in planta. Transcript levels of both AtTIPs were influenced by nitrogen supply but did not follow those of the nitrogen-derepressed ammonium transporter gene AtAMT1;1. Transgenic Arabidopsis plants overexpressing AtTIP2;1 did not show altered ammonium accumulation in roots after ammonium supply, although AtTIP2;1 mRNA levels in wild-type plants were up-regulated under these conditions. This study shows that AtTIP2;1 and AtTIP2;3 can mediate the extracytosolic transport of methyl-NH2 and NH3 across the tonoplast membrane and may thus participate in vacuolar ammonium compartmentation.
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