ArticlePlant physiology2005
Beyond complementation. Map-based cloning in Chlamydomonas reinhardtii.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- Identification of a gene controlling levels of the copper response regulator 1 transcription factor in Chlamydomonas reinhardtii.The Plant cell · 2024Article
- The Chlamydomonas Genome Project, version 6: Reference assemblies for mating-type plus and minus strains reveal extensive structural mutation in the laboratory.The Plant cell · 2023Article
- Co-expression networks in Chlamydomonas reveal significant rhythmicity in batch cultures and empower gene function discovery.The Plant cell · 2021Article
- An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas.Nature communications · 2020Article
- Cold Adaptation Mechanisms of a Snow AlgaFrontiers in microbiology · 2020Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Building a multipurpose insertional mutant library for forward and reverse genetics inPlant methods · 2017Article
- A Forward Genetic Screen and Whole Genome Sequencing Identify Deflagellation Defective Mutants in Chlamydomonas, Including Assignment of ADF1 as a TRP Channel.G3 (Bethesda, Md.) · 2016Article
- Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid Verde Fluorescent Protein as a reporter.Biotechnology journal · 2015Article
- Spontaneous dominant mutations in chlamydomonas highlight ongoing evolution by gene diversification.The Plant cell · 2015Article
- Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.Methods in cell biology · 2015Article
- The Chlamydomonas genome project: a decade on.Trends in plant science · 2014Review
- Thylakoid FtsH protease contributes to photosystem II and cytochrome b6f remodeling in Chlamydomonas reinhardtii under stress conditions.The Plant cell · 2014Article
- Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii.Biotechnology for biofuels · 2013Article
- Chlamydomonas ODA10 is a conserved axonemal protein that plays a unique role in outer dynein arm assembly.Molecular biology of the cell · 2013Article
- Species and population level molecular profiling reveals cryptic recombination and emergent asymmetry in the dimorphic mating locus of C. reinhardtii.PLoS genetics · 2013Article
- CDKL5 regulates flagellar length and localizes to the base of the flagella in Chlamydomonas.Molecular biology of the cell · 2013Article
- Mutations in axonemal dynein assembly factor DNAAF3 cause primary ciliary dyskinesia.Nature genetics · 2012Article
- Genome-wide characterization of genetic variation in the unicellular, green alga Chlamydomonas reinhardtii.PloS one · 2012Article
- Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydomonas reinhardtii is an excellent model system for plant biologists because of its ease of manipulation, facile genetics, and the ability to transform the nuclear, chloroplast, and mitochondrial genomes. Numerous forward genetics studies have been performed in Chlamydomonas, in many cases to elucidate the regulation of photosynthesis. One of the resultant challenges is moving from mutant phenotype to the gene mutation causing that phenotype. To date, complementation has been the primary method for gene cloning, but this is impractical in several situations, for example, when the complemented strain cannot be readily selected or in the case of recessive suppressors that restore photosynthesis. New tools, including a molecular map consisting of 506 markers and an 8X-draft nuclear genome sequence, are now available, making map-based cloning increasingly feasible. Here we discuss advances in map-based cloning developed using the strains mcd4 and mcd5, which carry recessive nuclear suppressors restoring photosynthesis to chloroplast mutants. Tools that have not been previously applied to Chlamydomonas, such as bulked segregant analysis and marker duplexing, are being implemented to increase the speed at which one can go from mutant phenotype to gene. In addition to assessing and applying current resources, we outline anticipated future developments in map-based cloning in the context of the newly extended Chlamydomonas genome initiative.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.