ArticleBiophysical journal2005
Surface changes of the mechanosensitive channel MscS upon its activation, inactivation, and closing.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment.International journal of molecular sciences · 2022Review
- Cyclodextrins increase membrane tension and are universal activators of mechanosensitive channels.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The mechanosensitive channel YbdG fromThe Journal of biological chemistry · 2019Article
- Voltage-Dependent Inactivation of MscS Occurs Independently of the Positively Charged Residues in the Transmembrane Domain.BioMed research international · 2016Article
- The evolutionary 'tinkering' of MscS-like channels: generation of structural and functional diversity.Pflugers Archiv : European journal of physiology · 2015Review
- Article
- The cytoplasmic cage domain of the mechanosensitive channel MscS is a sensor of macromolecular crowding.The Journal of general physiology · 2014Article
- Energetics of gating MscS by membrane tension in azolectin liposomes and giant spheroplasts.Channels (Austin, Tex.) · 2014Article
- A computational kinetic model of diffusion for molecular systems.The Journal of chemical physics · 2013Article
- MscS-like mechanosensitive channels in plants and microbes.Biochemistry · 2013Review
- The mechanoelectrical response of the cytoplasmic membrane of Vibrio cholerae.The Journal of general physiology · 2013Article
- Functional analysis of conserved motifs in the mechanosensitive channel homolog MscS-Like2 from Arabidopsis thaliana.PloS one · 2012Article
- Entropic tension in crowded membranes.PLoS computational biology · 2012Article
- Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes.The Journal of general physiology · 2011Article
- Mechanosensitive channels: what can they do and how do they do it?Structure (London, England : 1993) · 2011Review
- Genetic screen for potassium leaky small mechanosensitive channels (MscS) in Escherichia coli: recognition of cytoplasmic β domain as a new gating element.The Journal of biological chemistry · 2011Article
- Mechanosensitivity of ion channels based on protein-lipid interactions.Journal of the Royal Society, Interface · 2010Review
- Adaptive behavior of bacterial mechanosensitive channels is coupled to membrane mechanics.The Journal of general physiology · 2010Article
- The tension-transmitting 'clutch' in the mechanosensitive channel MscS.Nature structural & molecular biology · 2010Article
- Structural changes in the cytoplasmic domain of the mechanosensitive channel MscS during opening.Biophysical journal · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MscS is a bacterial mechanosensitive channel that shows voltage dependence. The crystal structure of MscS revealed that the channel is a homoheptamer with a large chamber on the intracellular site. Our previous experiments indicated that the cytoplasmic chamber of the channel is not a rigid structure and changes its conformation upon the channel activation. In this study, we have applied various sized cosolvents that are excluded from protein surfaces. It is well known that such cosolvents induce compaction of proteins and prevent thermal fluctuations. It is also known that they shift channel equilibrium to the state of lower volume. We have found that large cosolvents that cannot enter the channel interior accelerate channel inactivation when applied from the cytoplasmic side, but they slow down inactivation when applied from the extracellular side. We have also found that small cosolvents that can enter the channel cytoplasmic chamber prevent the channel from opening, unlike the large ones. These data support our idea that the channel cytoplasmic chamber shrinks upon inactivation but also give new clues about conformational changes of the channel upon transitions between its functional states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.