ArticleBiophysical journal2005
Probing the viscoelastic behavior of cultured airway smooth muscle cells with atomic force microscopy: stiffening induced by contractile agonist.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 218 citations in OpenAlex.
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- Characteristic frequencies of localized stress relaxation in scaling-law rheology of living cells.Biophysical journal · 2025Article
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
- Scaling-law mechanical marker for liver fibrosis diagnosis and drug screening through machine learning.Frontiers in bioengineering and biotechnology · 2024Article
- Lamellipodia dynamics and microrheology in endothelial cell paracellular gap closure.Biophysical journal · 2023Article
- Viscoelastic properties of suspended cells measured with shear flow deformation cytometry.eLife · 2022Article
- Frequency-dependent transition in power-law rheological behavior of living cells.Science advances · 2022Article
- Measuring Cytoskeletal Mechanical Fluctuations and Rheology with Active Micropost Arrays.Current protocols · 2022Article
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- A hierarchical cellular structural model to unravel the universal power-law rheological behavior of living cells.Nature communications · 2021Article
- Molecular Nanomechanical Mapping of Histamine-Induced Smooth Muscle Cell Contraction and Shortening.ACS nano · 2021Article
- Rheology of rounded mammalian cells over continuous high-frequencies.Nature communications · 2021Article
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- The plastic cell: mechanical deformation of cells and tissues.Open biology · 2021Review
- Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy.Beilstein journal of nanotechnology · 2021Article
- Cytoskeletal organization in isolated plant cells under geometry control.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Double power-law viscoelastic relaxation of living cells encodes motility trends.Scientific reports · 2020Article
- Differential Contributions of Actin and Myosin to the Physical Phenotypes and Invasion of Pancreatic Cancer Cells.Cellular and molecular bioengineering · 2020Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complex rheology of airway smooth muscle cells and its dynamic response during contractile stimulation involves many molecular processes, foremost of which are actomyosin cross-bridge cycling and actin polymerization. With an atomic force microscope, we tracked the spatial and temporal variations of the viscoelastic properties of cultured airway smooth muscle cells. Elasticity mapping identified stiff structural elements of the cytoskeletal network. Using a precisely positioned microscale probe, picoNewton forces and nanometer level indentation modulations were applied to cell surfaces at frequencies ranging from 0.5 to 100 Hz. The resulting elastic storage modulus (G') and dissipative modulus (G'') increased dramatically, with hysteresivity (eta = G''/G') showing a definitive decrease after stimulation with the contractile agonist 5-hydroxytryptamine. Frequency-dependent assays showed weak power-law structural damping behavior and universal scaling in support of the soft-glassy material description of cellular biophysics. Additionally, a high-frequency component of the loss modulus (attributed to cellular Newtonian viscosity) increased fourfold during the contractile process. The complex shear modulus showed a strong sensitivity to the degree of actin polymerization. Inhibitors of myosin light chain kinase activity had little effect on the stiffening response to contractile stimulation. Thus, our measurements appear to be particularly well suited for characterization of dynamic actin rheology during airway smooth muscle contraction.
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