ArticleInfection and immunity2005
HtrA stress protein is involved in intramacrophagic replication of adherent and invasive Escherichia coli strain LF82 isolated from a patient with Crohn's disease.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 98 citations in OpenAlex.
- The assembly of a hybrid type IV secretion system by a Crohn's disease-associated Escherichia coli strain.Nature communications · 2025Article
- Phenotypic characterization and complete genome of a tumorigenic pathobiont Escherichia coli LI60C3.Gut pathogens · 2025Article
- Persistence phenotype of adherent-invasiveMicrobial cell (Graz, Austria) · 2025Article
- Characterization and immunoprotection of thioredoxin reductase TrxB knockout mutant ofFrontiers in cellular and infection microbiology · 2025Article
- Outer membrane barrier impairment byMicrobiology (Reading, England) · 2024Article
- The CpxRA two-component system of adherent and invasiveInfection and immunity · 2024Article
- Contribution of Toxin-Antitoxin Systems to Adherent-InvasiveMicroorganisms · 2024Review
- Characterizing the Pathogenic Potential of Crohn's Disease-Associated Adherent-InvasiveEcoSal Plus · 2023Review
- Response mechanisms to acid stress promote LF82 replication in macrophages.Frontiers in cellular and infection microbiology · 2023Article
- Phenotypicand Genotypic Characterization of Clinical Isolates of Intracellular Adherent-InvasiveFrontiers in cellular and infection microbiology · 2022Article
- Autoimmune susceptibility geneGut · 2022Article
- The Crohn's disease-related bacterial strain LF82 assembles biofilm-like communities to protect itself from phagolysosomal attack.Communications biology · 2021Article
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- Commonality of adherent-invasive Escherichia coli isolated from patients with extraintestinal infections, healthy individuals and the environment.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Lipopolysaccharide O structure of adherent and invasive Escherichia coli regulates intestinal inflammation via complement C3.PLoS pathogens · 2020Article
- Characterization of mucosa-associated Escherichia coli strains isolated from Crohn's disease patients in Brazil.BMC microbiology · 2020Article
- Adherent-InvasiveInternational journal of molecular sciences · 2020Review
- Adaptation of adherent-invasiveGut microbes · 2020Article
- Why the discovery of adherent-invasiveWorld journal of biological chemistry · 2020Review
- The role of major virulence factors and pathogenicity of adherent-invasivePrzeglad gastroenterologiczny · 2020Review
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Adherent and invasive Escherichia coli (AIEC) bacteria isolated from Crohn's disease patients are able to greatly replicate within macrophages without escaping from the phagosome and without inducing macrophage death. In the present study, evidence is provided that in AIEC strain LF82 the htrA gene encoding the stress protein HtrA is essential for intracellular replication within J774-A1 macrophages. Deletion of the htrA gene in strain LF82 induced increased sensitivity of the isogenic mutant to oxidative stress caused by hydrogen peroxide and a reduced rate of growth in an acid and nutrient-poor medium partly reproducing the microenvironment of the phagosome. In vitro experiments using an LF82 htrA gene promoter fusion with the lacZ gene revealed a 38-fold activation of the promoter in AIEC LF82 intramacrophagic bacteria. The CpxRA two-component signaling pathway was not involved in this activation. In addition, the activation of the LF82 htrA gene promoter was not observed in the nonpathogenic E. coli K-12 intramacrophagic bacteria, indicating that the AIEC LF82 genetic background is crucial for induction of htrA gene transcription during phagocytosis.
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