ArticlePlant cell reports2005
Gene silencing studies in the gymnosperm species Pinus radiata.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- Strategies for Engineering Reproductive Sterility in Plantation Forests.Frontiers in plant science · 2018Article
- Biosynthesis and Metabolic Fate of Phenylalanine in Conifers.Frontiers in plant science · 2016Review
- Suppression of CCR impacts metabolite profile and cell wall composition in Pinus radiata tracheary elements.Plant molecular biology · 2013Article
- Suppression of 4-coumarate-CoA ligase in the coniferous gymnosperm Pinus radiata.Plant physiology · 2009Article
- Regeneration of transgenic Cryptomeria japonica D. Don after Agrobacterium tumefaciens-mediated transformation of embryogenic tissue.Plant cell reports · 2008Article
- Efficient and stable transgene suppression via RNAi in field-grown poplars.Transgenic research · 2008Article
- Testing of a heterologous, wound- and insect-inducible promoter for functional genomics studies in conifer defense.Plant cell reports · 2007Article
- Germin-like genes are expressed during somatic embryogenesis and early development of conifers.Plant molecular biology · 2006Article
- Enabling the study of gene function in gymnosperms: Virus-induced gene silencing inApplications in plant sciencesArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat. The effect of the different constructs on silencing the endogenous CAD gene was monitored in embryogenic tissue and somatic seedlings of 28 P. radiata transclones. Quantitative CAD measurements demonstrated that the construct containing an inverted-repeat of the CAD cDNA was most efficient in triggering gene silencing in P. radiata. Northern hybridization experiments with silenced transclones revealed that reduced CAD activities were the result of reduced steady state levels of the targeted CAD mRNA. Monitoring of the activity of the UBI1-promoter in the P. radiata transclones and heat-shock experiments with transgenic somatic P. radiata seedlings indicated that gene silencing is positively correlated with the expression level of the transgene. The obtained data are also consistent with a role for the expression level of the endogenous CAD gene in gene silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.